ArticlePLoS genetics2006
Drosophila SPF45: a bifunctional protein with roles in both splicing and DNA repair.
Article in PLoS genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Mechanism of Musashi2 affecting radiosensitivity of lung cancer by modulating DNA damage repair.MedComm · 2024Article
- Alternative splicing regulation of cell-cycle genes by SPF45/SR140/CHERP complex controls cell proliferation.RNA (New York, N.Y.) · 2021Article
- Xio is a component of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Downregulation of RNA binding motif protein 17 expression inhibits proliferation of hypopharyngeal carcinoma FaDu cells.Oncology letters · 2018Article
- The mNature communications · 2017Article
- Promiscuity in post-transcriptional control of gene expression: Drosophila sex-lethal and its regulatory partnerships.FEBS letters · 2017Review
- spenito is required for sex determination in Drosophila melanogaster.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Regulators of homologous recombination repair as novel targets for cancer treatment.Frontiers in genetics · 2015Review
- Splicing of receptor-like kinase-encoding SNC4 and CERK1 is regulated by two conserved splicing factors that are required for plant immunity.Molecular plant · 2014Article
- The Drosophila splicing factor PSI is phosphorylated by casein kinase II and tousled-like kinase.PloS one · 2013Article
- Mitogen-activated protein kinase phosphorylation of splicing factor 45 (SPF45) regulates SPF45 alternative splicing site utilization, proliferation, and cell adhesion.Molecular and cellular biology · 2012Article
- Regulation of chemoresistance via alternative messenger RNA splicing.Biochemical pharmacology · 2012Review
- The spliceosome-activating complex: molecular mechanisms underlying the function of a pleiotropic regulator.Frontiers in plant science · 2012Article
- Sex determination in insects: a binary decision based on alternative splicing.Current opinion in genetics & development · 2011Review
- PPS, a large multidomain protein, functions with sex-lethal to regulate alternative splicing in Drosophila.PLoS genetics · 2010Article
- Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes.Molecular and cellular biology · 2009Article
- Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1.Nature · 2008Article
- Functioning of the Drosophila Wilms'-tumor-1-associated protein homolog, Fl(2)d, in Sex-lethal-dependent alternative splicing.Genetics · 2008Article
- Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism.Nucleic acids research · 2007Article
- Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The sequence of the SPF45 protein is significantly conserved, yet functional studies have identified it as a splicing factor in animal cells and as a DNA-repair protein in plants. Using a combined genetic and biochemical approach to investigate this apparent functional discrepancy, we unify and validate both of these studies by demonstrating that the Drosophila melanogaster protein is bifunctional, with independent functions in DNA repair and splicing. We find that SPF45 associates with the U2 snRNP and that mutations that remove the C-terminal end of the protein disrupt this interaction. Although animals carrying this mutation are viable, they are nevertheless compromised in their ability to regulate Sex-lethal splicing, demonstrating that Sex-lethal is an important physiological target of SPF45. Furthermore, these mutant animals exhibit phenotypes diagnostic of difficulties in recovering from exogenously induced DNA damage. The conclusion that SPF45 functions in the DNA-repair pathway is strengthened by finding both genetic and physical interactions between SPF45 and RAD201, a previously uncharacterized member of the RecA/Rad51 protein family. Together with our finding that the fly SPF45 protein increases the survival rate of mutagen-treated bacteria lacking the RecG helicase, these studies provide the tantalizing suggestion that SPF45 has an ancient and evolutionarily conserved role in DNA repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.