ArticleMolecular biology of the cell2007
Negative regulation of yeast Eps15-like Arp2/3 complex activator, Pan1p, by the Hip1R-related protein, Sla2p, during endocytosis.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- Clathrin adaptors drive phase separation in endocytosis and trafficking.Communications biology · 2026Article
- Endocytosis and trans-Golgi Network in Yeast.Sub-cellular biochemistry · 2026Review
- Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway.The Journal of cell biology · 2022Article
- Candida albicansMicrobiology spectrum · 2022Article
- Cooperative regulation of endocytic vesicle transport by yeast Eps15-like protein Pan1p and epsins.The Journal of biological chemistry · 2021Article
- Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding.Nature communications · 2021Article
- MoEnd3 regulates appressorium formation and virulence through mediating endocytosis in rice blast fungus Magnaporthe oryzae.PLoS pathogens · 2017Article
- Identifying protein kinase-specific effectors of the osmostress response in yeast.Science signaling · 2017Article
- A Pan1/End3/Sla1 complex links Arp2/3-mediated actin assembly to sites of clathrin-mediated endocytosis.Molecular biology of the cell · 2015Article
- Pan1 regulates transitions between stages of clathrin-mediated endocytosis.Molecular biology of the cell · 2015Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Quantification of cytosolic interactions identifies Ede1 oligomers as key organizers of endocytosis.Molecular systems biology · 2014Article
- Regulation of clathrin-mediated endocytosis by dynamic ubiquitination and deubiquitination.Current biology : CB · 2014Article
- Zooming in on the molecular mechanisms of endocytic budding by time-resolved electron microscopy.Cellular and molecular life sciences : CMLS · 2014Review
- Targeted disruption of an EH-domain protein endocytic complex, Pan1-End3.Traffic (Copenhagen, Denmark) · 2014Article
- SLAC, a complex between Sla1 and Las17, regulates actin polymerization during clathrin-mediated endocytosis.Molecular biology of the cell · 2012Article
- Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis.Journal of cell science · 2012Article
- Validation of regulated protein phosphorylation events in yeast by quantitative mass spectrometry analysis of purified proteins.Proteomics · 2012Article
- Myosin 1E coordinates actin assembly and cargo trafficking during clathrin-mediated endocytosis.Molecular biology of the cell · 2012Article
- Regulation of clathrin coat assembly by Eps15 homology domain-mediated interactions during endocytosis.Molecular biology of the cell · 2012Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Control of actin assembly nucleated by the Arp2/3 complex plays a crucial role during budding yeast endocytosis. The yeast Eps15-related Arp2/3 complex activator, Pan1p, is essential for endocytic internalization and proper actin organization. Pan1p activity is negatively regulated by Prk1 kinase phosphorylation after endocytic internalization. Phosphorylated Pan1p is probably then dephosphorylated in the cytosol. Pan1p is recruited to endocytic sites approximately 25 s before initiation of actin polymerization, suggesting that its Arp2/3 complex activation activity is kept inactive during early stages of endocytosis by a yet-to-be-identified mechanism. However, how Pan1p is maintained in an inactive state is not clear. Using tandem affinity purification-tagged Pan1p, we identified End3p as a stoichiometric component of the Pan1p complex, and Sla2p, a yeast Hip1R-related protein, as a novel binding partner of Pan1p. Interestingly, Sla2p specifically inhibited Pan1p Arp2/3 complex activation activity in vitro. The coiled-coil region of Sla2p was important for Pan1p inhibition, and a pan1 partial loss-of-function mutant suppressed the temperature sensitivity, endocytic phenotypes, and actin phenotypes observed in sla2DeltaCC mutant cells that lack the coiled-coil region. Overall, our results establish that Sla2p's regulation of Pan1p plays an important role in controlling Pan1p-stimulated actin polymerization during endocytosis.
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