ArticleMolecular biology of the cell2007
Transcriptional silencing of a novel hTERT reporter locus during in vitro differentiation of mouse embryonic stem cells.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- TERT-CLPTM1L polymorphism rs401681 contributes to cancers risk: evidence from a meta-analysis based on 29 publications.PloS one · 2012Pooled it
- TERT promoter C228T mutation in neural progenitors confers growth advantage following telomere shortening in vivo.Neuro-oncology · 2022Article
- Targeting telomeres: advances in telomere maintenance mechanism-specific cancer therapies.Nature reviews. Cancer · 2022Review
- Polymorphic tandem DNA repeats activate the human telomerase reverse transcriptase gene.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Engineering a humanized telomerase reverse transcriptase gene in mouse embryonic stem cells.Scientific reports · 2019Article
- Diverse regulatory manners of human telomerase reverse transcriptase.Cell communication and signaling : CCS · 2019Review
- HoxC5 and miR-615-3p target newly evolved genomic regions to repress hTERT and inhibit tumorigenesis.Nature communications · 2018Article
- Regulation of human and mouse telomerase genes by genomic contexts and transcription factors during embryonic stem cell differentiation.Scientific reports · 2017Article
- Repression of telomerase gene promoter requires human-specific genomic context and is mediated by multiple HDAC1-containing corepressor complexes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum.Planta · 2017Article
- Review
- Therapeutic targeting of replicative immortality.Seminars in cancer biology · 2015Review
- Association of CRR9 locus with elevated risk of squamous cell carcinoma and basal cell carcinoma.International journal of clinical and experimental medicine · 2015Article
- Dual roles of c-Myc in the regulation of hTERT gene.Nucleic acids research · 2014Article
- Telomerase regulation.Mutation research · 2012Review
- A BAC transgenic reporter recapitulates in vivo regulation of human telomerase reverse transcriptase in development and tumorigenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- A multi-step strategy for BAC recombineering of large DNA fragments.International journal of biochemistry and molecular biology · 2011Article
- p16(INK4a) -mediated suppression of telomerase in normal and malignant human breast cells.Aging cell · 2010Article
- Evaluation of combinatorial cis-regulatory elements for stable gene expression in chicken cells.BMC biotechnology · 2010Article
- Robust activation of the human but not mouse telomerase gene during the induction of pluripotency.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The human telomerase reverse transcriptase hTERT is highly expressed in undifferentiated embryonic cells and silenced in the majority of somatic cells. To investigate the mechanisms of hTERT silencing, we have developed a novel reporter using a bacterial artificial chromosome (BAC) that contained the entire hTERT gene and its neighboring loci, hCRR9 and hXtrp2. Firefly and Renilla luciferases were used to monitor transcription from the hTERT and hCRR9 promoters, respectively. In mouse embryonic stem cells stably integrated with the BAC reporter, both hTERT and hCRR9 promoters were highly expressed. Upon differentiation into embryoid bodies and further into mineral-producing osteogenic cells, the hTERT promoter activity decreased progressively, whereas the hCRR9 promoter remained highly active, both resembling their endogenous counterparts. In fully differentiated cells, the hTERT promoter was completely silenced and adopted a chromatin structure that was similar to its native counterpart in human cells. Inhibition of histone deacetylases led to the opening of the hTERT promoter and partially relieved repression, suggesting that histone deacetylation was necessary but not sufficient for hTERT silencing. Thus, our result demonstrated that developmental silencing of the human TERT locus could be recapitulated in a chromosomal position-independent manner during the differentiation of mouse embryonic stem cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.