ArticleGenetics2007
The overexpression of a Saccharomyces cerevisiae centromeric histone H3 variant mutant protein leads to a defect in kinetochore biorientation.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Cell cycle dependent methylation of Dam1 contributes to kinetochore integrity and faithful chromosome segregation.PLoS genetics · 2025Article
- Humanization reveals pervasive incompatibility of yeast and human kinetochore components.G3 (Bethesda, Md.) · 2023Article
- Molecular Dynamics and Evolution of Centromeres in the Genus Equus.International journal of molecular sciences · 2022Review
- Review
- Dbf4-Dependent Kinase (DDK)-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A inG3 (Bethesda, Md.) · 2020Article
- Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast.Nucleic acids research · 2018Article
- Article
- Budding yeast CENP-ACell cycle (Georgetown, Tex.) · 2018Article
- Chromatin dynamics during the cell cycle at centromeres.Nature reviews. Genetics · 2017Review
- Regulation of Budding Yeast CENP-A levels Prevents Misincorporation at Promoter Nucleosomes and Transcriptional Defects.PLoS genetics · 2016Article
- Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.PLoS genetics · 2016Article
- SAGA DUB-Ubp8 Deubiquitylates Centromeric Histone Variant Cse4.G3 (Bethesda, Md.) · 2015Article
- Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein.The Journal of biological chemistry · 2014Article
- Checkpoints couple transcription network oscillator dynamics to cell-cycle progression.Genome biology · 2014Article
- Evolutionary insights into the role of the essential centromere protein CAL1 in Drosophila.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Misregulation of Scm3p/HJURP causes chromosome instability in Saccharomyces cerevisiae and human cells.PLoS genetics · 2011Article
- New clues to understand how CENP-A maintains centromere identity.Cell division · 2011Article
- Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 and HIR proteins in Saccharomyces cerevisiae.Genetics · 2011Article
- Efficient yeast ChIP-Seq using multiplex short-read DNA sequencing.BMC genomics · 2009Article
- Condensin function at centromere chromatin facilitates proper kinetochore tension and ensures correct mitotic segregation of sister chromatids.Genes to cells : devoted to molecular & cellular mechanisms · 2007Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosomes segregate using their kinetochores, the specialized protein structures that are assembled on centromeric DNA and mediate attachment to the mitotic spindle. Because centromeric sequences are not conserved, centromere identity is propagated by an epigenetic mechanism. All eukaryotes contain an essential histone H3 variant (CenH3) that localizes exclusively to centromeres. Because CenH3 is required for kinetochore assembly and is likely to be the epigenetic mark that specifies centromere identity, it is critical to elucidate the mechanisms that assemble and maintain CenH3 exclusively at centromeres. To learn more about the functions and regulation of CenH3, we isolated mutants in the budding yeast CenH3 that are lethal when overexpressed. These CenH3 mutants fall into three unique classes: (I) those that localize to euchromatin but do not alter kinetochore function, (II) those that localize to the centromere and disrupt kinetochore function, and (III) those that no longer target to the centromere but still disrupt chromosome segregation. We found that a class III mutant is specifically defective in the ability of sister kinetochores to biorient and attach to microtubules from opposite spindle poles, indicating that CenH3 mutants defective in kinetochore biorientation can be obtained.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.