ArticleGenetics2007
Onset of the DNA replication checkpoint in the early Drosophila embryo.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Topological interactions drive the first fate decision in theNature physics · 2025Article
- Confinement promotes nematic alignment of spindle-shaped cells during Drosophila embryogenesis.Development (Cambridge, England) · 2024Article
- The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation.Nature communications · 2021Article
- Inducing controlled cell cycle arrest and re-entry during asexual proliferation of Plasmodium falciparum malaria parasites.Scientific reports · 2018Article
- Rapid DNA Synthesis During EarlyGenetics · 2017Article
- Timing the Drosophila Mid-Blastula Transition: A Cell Cycle-Centered View.Trends in genetics : TIG · 2016Review
- Zygotic genome activation triggers the DNA replication checkpoint at the midblastula transition.Cell · 2015Article
- The relative ages of eukaryotes and akaryotes.Journal of molecular evolution · 2014Article
- Article
- DNA replication times the cell cycle and contributes to the mid-blastula transition in Drosophila embryos.The Journal of cell biology · 2009Article
- Coupling of zygotic transcription to mitotic control at the Drosophila mid-blastula transition.Development (Cambridge, England) · 2009Article
- Influence of cyclin type and dose on mitotic entry and progression in the early Drosophila embryo.The Journal of cell biology · 2009Article
- An essential role for the RNA-binding protein Smaug during the Drosophila maternal-to-zygotic transition.Development (Cambridge, England) · 2009Article
- no poles encodes a predicted E3 ubiquitin ligase required for early embryonic development of Drosophila.Development (Cambridge, England) · 2009Article
- Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation.The Journal of cell biology · 2008Article
- RNAi of mitotic cyclins in Drosophila uncouples the nuclear and centrosome cycle.Current biology : CB · 2008Article
- Stem-loop binding protein expressed in growing oocytes is required for accumulation of mRNAs encoding histones H3 and H4 and for early embryonic development in the mouse.Developmental biology · 2008Article
- A precise Bicoid gradient is nonessential during cycles 11-13 for precise patterning in the Drosophila blastoderm.PloS one · 2008Article
- SMK-1/PPH-4.1-mediated silencing of the CHK-1 response to DNA damage in early C. elegans embryos.The Journal of cell biology · 2007Article
- Reducing DNA polymerase alpha in the absence of Drosophila ATR leads to P53-dependent apoptosis and developmental defects.Genetics · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila embryo is a promising model for isolating gene products that coordinate S phase and mitosis. We have reported before that increasing maternal Cyclin B dosage to up to six copies (six cycB) increases Cdk1-Cyclin B (CycB) levels and activity in the embryo, delays nuclear migration at cycle 10, and produces abnormal nuclei at cycle 14. Here we show that the level of CycB in the embryo inversely correlates with the ability to lengthen interphase as the embryo transits from preblastoderm to blastoderm stages and defines the onset of a checkpoint that regulates mitosis when DNA replication is blocked with aphidicolin. A screen for modifiers of the six cycB phenotypes identified 10 new suppressor deficiencies. In addition, heterozygote dRPA2 (a DNA replication gene) mutants suppressed only the abnormal nuclear phenotype at cycle 14. Reduction of dRPA2 also restored interphase duration and checkpoint efficacy to control levels. We propose that lowered dRPA2 levels activate Grp/Chk1 to counteract excess Cdk1-CycB activity and restore interphase duration and the ability to block mitosis in response to aphidicolin. Our results suggest an antagonistic interaction between DNA replication checkpoint activation and Cdk1-CycB activity during the transition from preblastoderm to blastoderm cycles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.