ArticleGenetics2007
Generating novel allelic variation through Activator insertional mutagenesis in maize.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Toward Transgene-Free Transposon-Mediated Biological Mutagenesis for Plant Breeding.International journal of molecular sciences · 2023Review
- ThePlant direct · 2022Article
- De novo screening of disease-resistant genes from the chromosome-level genome of rare minnow using CRISPR-cas9 random mutation.GigaScience · 2021Article
- Development of an activation tagging system for maize.Plant direct · 2019Article
- Characterization and Transposon Mutagenesis of the Maize (Zea mays) Pho1 Gene Family.PloS one · 2016Article
- Characterization of an Ac transposon system based on apt1-m1 (Ac) on the long arm of maize chromosome 9.Genetica · 2012Article
- Transposition behavior of nonautonomous a hAT superfamily transposon nDart in rice (Oryza sativa L.).Molecular genetics and genomics : MGG · 2011Article
- Article
- An Ac transposon system based on maize chromosome 4S for isolating long-distance-transposed Ac tags in the maize genome.Genetica · 2010Article
- Genome-wide distribution of transposed Dissociation elements in maize.The Plant cell · 2010Article
- Fusion of reverse-oriented Ds termini following abortive transposition in Arabidopsis: implications for the mechanism of Ac/Ds transposition.Plant cell reports · 2010Article
- Genetic resources for maize cell wall biology.Plant physiology · 2009Article
- Transposon excision from an atypical site: a mechanism of evolution of novel transposable elements.PloS one · 2007Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maize transposable element Activator (Ac) has been exploited as an insertional mutagen to disrupt, clone, and characterize genes in a number of plant species. To develop an Ac-based mutagenesis platform for maize, a large-scale mutagenesis was conducted targeting the pink scutellum1 locus. We selected 1092 Ac transposition events from a closely linked donor Ac, resulting in the recovery of 17 novel ps1 alleles. Multiple phenotypic classes were identified corresponding to Ac insertions in the 5'-UTR and coding region of the predicted Ps1 gene. To generate a stable allelic series, we employed genetic screens and identified 83 germinally heritable ps1 excision alleles. Molecular characterization of these excision alleles revealed a position-dependent bias in excision allele frequencies and the predominance of 7- and 8-bp footprint products. In total, 19 unique ps1 excision alleles were generated in this study, including several that resulted in weak mutant phenotypes. The analysis of footprint alleles suggests a model of Ac excision in maize that is consistent with recent in vitro studies of hAT element excision. Importantly, the genetic and molecular methods developed in this study can be extended to generate novel allelic variation at any Ac-tagged gene in the genome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.