ArticleJournal of clinical microbiology2007
Detection and differentiation of Bordetella spp. by real-time PCR.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 38 citations in OpenAlex.
- Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock.BMC veterinary research · 2017Article
- Quantitative PCR and Cytology of Bronchoalveolar Lavage Fluid in Dogs with Bordetella bronchiseptica Infection.Journal of veterinary internal medicine · 2016Article
- Molecular and antimicrobial analyses of non-classical Bordetella isolated from a laboratory mouse.The Journal of veterinary medical science · 2016Article
- Protecting Newborns Against Pertussis: Treatment and Prevention Strategies.Paediatric drugs · 2015Review
- Laboratory Diagnosis of Pertussis.Clinical microbiology reviews · 2015Review
- Recommendation for a Standardised Method of Broth Microdilution Susceptibility Testing for Porcine Bordetella bronchiseptica.PloS one · 2015Article
- Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran.Iranian journal of microbiology · 2013Article
- Towards improved accuracy of Bordetella pertussis nucleic acid amplification tests.Journal of clinical microbiology · 2012Review
- Validation of laboratory-developed molecular assays for infectious diseases.Clinical microbiology reviews · 2010Review
- Bordetella bronchiseptica infection in cats. ABCD guidelines on prevention and management.Journal of feline medicine and surgery · 2009Review
- Preparation of Bordetella pertussis DNA from respiratory samples for real-time PCR by commercial kits.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2008Article
- Bordetella pertussis polymorphism and pertussis vaccines.Clinical and vaccine immunology : CVI · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular detection of Bordetella pertussis DNA is a sensitive and specific method for the rapid diagnosis of pertussis. In this study, a new molecular assay for the detection and differentiation of Bordetella spp. based on automated DNA extraction and real-time PCR was evaluated. The analytical sensitivity of the new assay was determined by Probit analysis of serial dilutions of both cloned PCR products IS481 and IS1001 and cell suspensions of B. pertussis, B. parapertussis, and B. bronchiseptica. The specificity was analyzed by testing a number of pathogens producing respiratory infections. Moreover, a total of 92 clinical samples were investigated. The results were compared to those obtained by an in-house assay based on manual DNA extraction, followed by real-time PCR and detection of IS481. The analytical sensitivity of the new assay for the detection of IS481 and IS1001 was determined to be 2.2 and 1.2 genome equivalents/mul, respectively. The analytical sensitivity for the detection of B. pertussis, B. parapertussis, and B. bronchiseptica was determined to be 1.6, 1.0, and 2.7 genome equivalents/mul, respectively. When clinical specimens were tested with the new assay, 46 of 92 were found to be positive for Bordetella DNA. With the in-house assay, 45 samples tested positive. The new molecular assay proved to be suitable for the rapid diagnosis of pertussis in the routine diagnostic laboratory.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.