ArticleJournal of clinical microbiology2007
Multitarget PCR for diagnosis of pertussis and its clinical implications.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Population-Based Pertussis Incidence and Risk Factors in Infants Less Than 6 Months in Nepal.Journal of the Pediatric Infectious Diseases Society · 2017Trial
- Designs of two randomized, community-based trials to assess the impact of influenza immunization during pregnancy on respiratory illness among pregnant women and their infants and reproductive outcomes in rural Nepal.BMC pregnancy and childbirth · 2015Trial
- A triple-target reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid and accurate detection of SARS-CoV-2 virus.Analytica chimica acta · 2023Article
- A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis.Scientific reports · 2023Article
- AsymptomaticeLife · 2021Observational
- AsymptomaticmedRxiv : the preprint server for health sciences · 2020Article
- Identification of etiologic agents and clinical characteristics for patients suspected of having pertussis in a large Children's Hospital in China.Annals of translational medicine · 2019Article
- Risk Factors Associated With Bordetella pertussis Among Infants ≤4 Months of Age in the Pre-Tdap Era: United States, 2002-2005.The Pediatric infectious disease journal · 2017Article
- The relationship between mucosal immunity, nasopharyngeal carriage, asymptomatic transmission and the resurgence ofF1000Research · 2017Review
- Comparison of molecular detection methods for pertussis in children during a state-wide outbreak.Annals of clinical microbiology and antimicrobials · 2016Article
- Protecting Newborns Against Pertussis: Treatment and Prevention Strategies.Paediatric drugs · 2015Review
- Laboratory Diagnosis of Pertussis.Clinical microbiology reviews · 2015Review
- Testing implications of varying targets for Bordetella pertussis: comparison of the FilmArray Respiratory Panel and the Focus B. pertussis PCR assay.Journal of clinical pathology · 2015Article
- Evaluation of amplification targets for the specific detection of Bordetella pertussis using real-time polymerase chain reaction.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2014Article
- Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.Iranian journal of microbiology · 2014Article
- Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran.Iranian journal of microbiology · 2013Article
- Detection of Bordetella pertussis from Clinical Samples by Culture and End-Point PCR in Malaysian Patients.International journal of bacteriology · 2013Article
- Towards improved accuracy of Bordetella pertussis nucleic acid amplification tests.Journal of clinical microbiology · 2012Review
- Identification and evaluation of new target sequences for specific detection of Bordetella pertussis by real-time PCR.Journal of clinical microbiology · 2008Article
- Pertussis vaccination for health care workers.Clinical microbiology reviews · 2008Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR has greatly facilitated pertussis diagnosis due to the speed, sensitivity, and specificity of this assay compared to other detection methods. Various single-target PCR assays are currently utilized, but none is universally considered to be the "gold standard." Our aim was to assess the use of multitarget versus single-target PCR for the diagnosis of pertussis in clinical samples. PCR assays targeting insertion sequence IS481 (IS), pertussis toxin ptxA promoter region (PT), and outer membrane porin (PO), or recA (RA) were evaluated in respiratory specimens collected from 4,442 patients with suspected pertussis. The diagnosis of pertussis was confirmed in 309 (6.96%) patients by the 3-target IS-PT-PO/RA PCR versus 247 (5.56%) by the conventional single-target IS (P=0.007). Compared to single-target IS, the three-target combination increased the proportion of positive specimens by 1.25-fold, and two-target combinations increased the proportion of positive specimens by 1.10- to 1.24-fold. In addition, nine cases of B. parapertussis infection were also confirmed by using the discriminative features of this multitarget PCR. Of the 89 culture-proven pertussis cases, 17 (19.1%) and 5 of the 16 patients (31.3%) admitted to intensive care unit would have been missed had only the single-target IS PCR been applied. Patients with mild disease (P=0.004) and shorter hospitalization (P=0.006) were less likely to have positive cultures. This consensus generating real-time PCR approach permits a sensitive detection, as well as an accurate species identification of the causative Bordetella pathogens for the timely management of patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.