ArticleNucleic acids research2007
An improved method to identify BAC clones using pooled overgos.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- History and future perspectives of barley genomics.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Review
- A sequence-ready physical map of barley anchored genetically by two million single-nucleotide polymorphisms.Plant physiology · 2014Article
- Genomic analysis reveals MATH gene(s) as candidate(s) for Plum pox virus (PPV) resistance in apricot (Prunus armeniaca L.).Molecular plant pathology · 2013Article
- Combinatorial pooling enables selective sequencing of the barley gene space.PLoS computational biology · 2013Article
- Article
- Advances in BAC-based physical mapping and map integration strategies in plants.Journal of biomedicine & biotechnology · 2012Review
- Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region.Molecular plant pathology · 2011Article
- De novo 454 sequencing of barcoded BAC pools for comprehensive gene survey and genome analysis in the complex genome of barley.BMC genomics · 2009Article
- A compartmentalized approach to the assembly of physical maps.BMC bioinformatics · 2009Article
- The international barley sequencing consortium--at the threshold of efficient access to the barley genome.Plant physiology · 2009Article
- Isolation, analysis and marker utility of novel miniature inverted repeat transposable elements from the barley genome.Molecular genetics and genomics : MGG · 2008Article
- Barley genomics: An overview.International journal of plant genomics · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hybridization using overgo probes is an established approach for screening arrayed bacterial artificial chromosome (BAC) libraries. We have improved the use of overgos by increasing the yield of positive clones using reduced levels of radioisotopes and enzyme. The strategy involves labeling with all four radiolabeled nucleotides in a hot pulse followed by a cold nucleotide chase and then extending the exposure time to compensate for reduced specific activity of the probes. The resulting cost savings and reduced human exposure to radiation make the use of highly pooled overgo probes a more attractive approach for screening of BAC libraries from organisms with large genomes.
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