ArticleEuropean urology2007
Diabetes-induced changes in the alternative splicing of the slo gene in corporal tissue.
Article in European urology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Coronary Large Conductance CaFrontiers in physiology · 2021Review
- Gene Therapy for Overactive Bladder: A Review of BK-Channel α-Subunit Gene Transfer.Therapeutics and clinical risk management · 2021Review
- Differential expression of neuregulin-1 isoforms and downregulation of erbin are associated with Erb B2 receptor activation in diabetic peripheral neuropathy.Acta neuropathologica communications · 2013Article
- Silencing MaxiK activity in corporal smooth muscle cells initiates compensatory mechanisms to maintain calcium homeostasis.The journal of sexual medicine · 2011Article
- Gene therapy for erectile dysfunction: what is the future?Current urology reports · 2010Review
- Membrane trafficking of large conductance calcium-activated potassium channels is regulated by alternative splicing of a transplantable, acidic trafficking motif in the RCK1-RCK2 linker.The Journal of biological chemistry · 2010Article
- Molecular targets for diabetes mellitus-associated erectile dysfunction.Molecular & cellular proteomics : MCP · 2010Article
- The opiorphin gene (ProL1) and its homologues function in erectile physiology.BJU international · 2008Article
- A mutually exclusive alternative exon of slo1 codes for a neuronal BK channel with altered function.Channels (Austin, Tex.)Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesErectile dysfunction is a common diabetic complication. Preclinical studies have documented that the Slo gene (encoding the BK or Maxi-K channel alpha-subunit) plays a critical role in erectile function. Therefore, we determined whether diabetes induces changes in the splicing of the Slo gene relevant to erectile function.
methodsReverse transcriptase-polymerase chain reaction was used to compare Slo splice variant expression in corporal tissue excised from control and streptozotocin (STZ)-induced diabetic Fischer F-344 rats. Splice variants were sequenced, characterized by patch clamping, and fused to green fluorescent protein to determine cellular localization. The impact of altered Slo expression on erectile function was further evaluated in vivo.
resultsA novel Slo splice variant (SVcyt, with a cytoplasmic location) was predominantly expressed in corporal tissue from control rats. STZ-diabetes caused upregulation of a channel-forming transcript SV0. Preliminary results suggest that SV0 was also more prevalent in the corporal tissue of human diabetic compared with nondiabetic patients. The change in isoform expression in STZ-treated rats was partially reversed by insulin treatment. Intracorporal injection of a plasmid expressing the SV0 transcript, but not SVcyt, restored erectile function in STZ-diabetic rats.
conclusionsAlternative splicing of the Slo transcript may represent an important compensatory mechanism to increase the ease with which relaxation of corporal tissue may be triggered as a result of a diabetes-related decline in erectile capacity.
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