ArticleThe Journal of cell biology1991
Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 98 citations in OpenAlex.
- The midbody component Prc1-like is required for microtubule reorganization during cytokinesis and dorsal determinant segregation in the early zebrafish embryo.Development (Cambridge, England) · 2023Article
- Cell cortex regulation by the planar cell polarity protein Prickle1.The Journal of cell biology · 2022Article
- Vertebrate Axial Patterning: From Egg to Asymmetry.Advances in experimental medicine and biology · 2017Review
- Kinesin-1 interacts with Bucky ball to form germ cells and is required to pattern the zebrafish body axis.Development (Cambridge, England) · 2015Article
- Cortical depth and differential transport of vegetally localized dorsal and germ line determinants in the zebrafish embryo.Bioarchitecture · 2014Article
- Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development.Gene expression patterns : GEP · 2013Article
- Maternal Dead-End1 is required for vegetal cortical microtubule assembly during Xenopus axis specification.Development (Cambridge, England) · 2013Article
- Vegetally localized Xenopus trim36 regulates cortical rotation and dorsal axis formation.Development (Cambridge, England) · 2009Article
- Endoplasmic reticulum positioning and partitioning in mitotic HeLa cells.Journal of anatomy · 2005Article
- Activation of myosin V-based motility and F-actin-dependent network formation of endoplasmic reticulum during mitosis.The Journal of cell biology · 2002Article
- Dynein supports motility of endoplasmic reticulum in the fungus Ustilago maydis.Molecular biology of the cell · 2002Article
- Clonal tests of conventional kinesin function during cell proliferation and differentiation.Molecular biology of the cell · 2000Article
- Microtubule-based endoplasmic reticulum motility in Xenopus laevis: activation of membrane-associated kinesin during development.Molecular biology of the cell · 1999Article
- Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution.The Journal of cell biology · 1997Article
- Microtubule-mediated transport of organelles and localization of beta-catenin to the future dorsal side of Xenopus eggs.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Protein phosphatase 1 regulates the cytoplasmic dynein-driven formation of endoplasmic reticulum networks in vitro.The Journal of cell biology · 1995Article
- Independence of two microtubule systems in fertilized frog eggs: the sperm aster and the vegetal parallel array.Roux's archives of developmental biology : the official organ of the EDBO · 1993Article
- Polarity and reorganization of the endoplasmic reticulum during fertilization and ooplasmic segregation in the ascidian egg.The Journal of cell biology · 1993Article
- Suppression of kinesin expression in cultured hippocampal neurons using antisense oligonucleotides.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the first cell cycle, the vegetal cortex of the fertilized frog egg is translocated over the cytoplasm. This process of cortical rotation creates regional cytoplasmic differences important in later development, and appears to involve an array of aligned microtubules that forms transiently beneath the vegetal cortex. We have investigated how these microtubules might be involved in generating movement by analyzing isolated cortices and sections of Xenopus laevis and Rana pipiens eggs. First, the polarity of the cortical microtubules was determined using the "hook" assay. Almost all microtubules had their plus ends pointing in the direction of cortical rotation. Secondly, the association of microtubules with other cytoplasmic elements was examined. Immunofluorescence revealed that cytokeratin filaments coalign with the microtubules. The timing of their appearance and their position on the cytoplasmic side of the microtubules suggested that they are not involved directly in generating movement. ER was visualized with the dye DiIC16(3) and by immunofluorescence with anti-BiP (Bole, D. G., L. M. Hendershot, and J. F. Kearney, 1986. J. Cell Biol. 102:1558-1566). One layer of ER was found closely underlying the plasma membrane at all times. An additional, deeper layer formed in association with the microtubules of the array. Antibodies to sea urchin kinesin (Ingold, A. L., S. A. Cohn, and J. M. Scholey. 1988. J. Cell Biol. 107:2657-2667) detected antigens associated with both the ER and microtubules. On immunoblots they recognized microtubule associated polypeptide(s) of approximately 115 kD from Xenopus eggs. These observations are consistent with a role for kinesin in creating movement between the microtubules and ER, which leads in turn to the cortical rotation.
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