ArticleBMC evolutionary biology2006
Increased susceptibility to repeated freeze-thaw cycles in Escherichia coli following long-term evolution in a benign environment.
Article in BMC evolutionary biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Article
- Revival of Mucin-Degrading Human-Derived Fecal Microbial Consortia After Cryopreservation.microPublication biology · 2026Article
- Set-up of a pharmaceutical cell bank of Magnetospirillum gryphiswaldense MSR1 magnetotactic bacteria producing highly pure magnetosomes.Microbial cell factories · 2024Article
- Survival of Icelandic airborne microbes towards simulated atmospheric stress factors.Extremophiles : life under extreme conditions · 2023Article
- Any Future for Faecal Microbiota Transplantation as a Novel Strategy for Gut Microbiota Modulation in Human and Veterinary Medicine?Life (Basel, Switzerland) · 2022Review
- The Effects of Freeze-Thaw and UVC Radiation on Microbial Survivability in a Selected Mars-like Environment.Microorganisms · 2022Article
- The CArchives of microbiology · 2021Article
- Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes.Archives of microbiology · 2021Article
- A standardised model for stool banking for faecal microbiota transplantation: a consensus report from a multidisciplinary UEG working group.United European gastroenterology journal · 2021Article
- Impact of Freeze-Thaw Cycles on Die-Off ofInternational journal of environmental research and public health · 2020Article
- Consequences of Cryopreservation in Diverse Natural Isolates of Saccharomyces cerevisiae.Genome biology and evolution · 2020Article
- Metagenomic Signatures of Gut Infections Caused by DifferentApplied and environmental microbiology · 2019Article
- A Glacier Bacterium Produces High Yield of Cryoprotective Exopolysaccharide.Frontiers in microbiology · 2019Article
- Article
- How Archiving by Freezing Affects the Genome-Scale Diversity of Escherichia coli Populations.Genome biology and evolution · 2016Article
- Review
- Molecular characterization and functional analysis by heterologous expression in E. coli under diverse abiotic stresses for OsLEA5, the atypical hydrophobic LEA protein from Oryza sativa L.Molecular genetics and genomics : MGG · 2012Article
- The cost of gene expression underlies a fitness trade-off in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Effect of sample storage on stability of salivary glutathione, lipid peroxidation levels, and tissue factor activity.Journal of clinical laboratory analysis · 2009Article
- Surviving the bottleneck: transmission mutants and the evolution of microbial populations.Genetics · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn order to study the dynamics of evolutionary change, 12 populations of E. coli B were serially propagated for 20,000 generations in minimal glucose medium at constant 37 degrees C. Correlated changes in various other traits have been previously associated with the improvement in competitive fitness in the selective environment. This study examines whether these evolved lines changed in their ability to tolerate the stresses of prolonged freezing and repeated freeze-thaw cycles during adaptation to a benign environment.
resultsAll 12 lines that evolved in the benign environment for 20,000 generations are more sensitive to freeze-thaw cycles than their ancestor. The evolved lines have an average mortality rate of 54% per daily cycle, compared to the ancestral rate of 34%. By contrast, there was no significant difference between the evolved lines and their ancestor in mortality during prolonged freezing. There was also some variability among the evolved lines in susceptibility to repeated freeze-thaw cycles. Those lines that had evolved higher competitive fitness in the minimal glucose medium at 37 degrees C also had higher mortality during freeze-thaw cycles. This variability was not associated, however, with differences among lines in DNA repair functionality and mutability.
conclusionThe consistency of the evolutionary declines in freeze-thaw tolerance, the correlation between fitness in glucose medium at 37 degrees C and mortality during freeze-thaw cycles, and the absence of greater declines in freeze-thaw survival among the hypermutable lines all indicate a trade-off between performance in minimal glucose medium at 37 degrees C and the capacity to tolerate this stress. Analyses of the mutations that enhance fitness at 37 degrees C may shed light on the physiological basis of this trade-off.
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