ArticlePlanta2007
In planta AKT2 subunits constitute a pH- and Ca2+-sensitive inward rectifying K+ channel.
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- New Insights into Plant TPK Ion Channel Evolution.Plants (Basel, Switzerland) · 2021Article
- HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll HPlant biotechnology journal · 2020Article
- Plant Membrane Transport Research in the Post-genomic Era.Plant communications · 2020Review
- High V-PPase activity is beneficial under high salt loads, but detrimental without salinity.The New phytologist · 2018Article
- Interaction between calcium and potassium modulates elongation rate in cotton fiber cells.Journal of experimental botany · 2017Article
- The receptor-like pseudokinase MRH1 interacts with the voltage-gated potassium channel AKT2.Scientific reports · 2017Article
- The Nonspecific Lipid Transfer Protein AtLtpI-4 Is Involved in Suberin Formation of Arabidopsis thaliana Crown Galls.Plant physiology · 2016Article
- The AKT2 potassium channel mediates NaCl induced depolarization in the root of Arabidopsis thaliana.Plant signaling & behavior · 2016Article
- Comparison between Arabidopsis and Rice for Main Pathways of K(+) and Na(+) Uptake by Roots.Frontiers in plant science · 2016Review
- Two fatty acid desaturases, STEAROYL-ACYL CARRIER PROTEIN Δ9-DESATURASE6 and FATTY ACID DESATURASE3, are involved in drought and hypoxia stress signaling in Arabidopsis crown galls.Plant physiology · 2014Article
- The role of K(+) channels in uptake and redistribution of potassium in the model plant Arabidopsis thaliana.Frontiers in plant science · 2013Article
- Assembly and sorting of the tonoplast potassium channel AtTPK1 and its turnover by internalization into the vacuole.Plant physiology · 2011Article
- Agroinfiltration of grapevine leaves for fast transient assays of gene expression and for long-term production of stable transformed cells.Plant cell reports · 2008Article
- A central role of abscisic acid in drought stress protection of Agrobacterium-induced tumors on Arabidopsis.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterologous expression of plant genes in yeast and animal cells represents a common approach to study plant ion channels. When expressed in Xenopus oocytes and COS cells the Arabidopsis Shaker-like K+ channel, AKT2 forms a weakly voltage-dependent channel, blocked by Ca2+ and protons. Channels with these characteristics, however, were not found in AKT2-expressing Arabidopsis cell types. To understand this phenomenon, we employed Agrobacterium-mediated transient transformation to functionally characterise Arabidopsis thaliana channels in Nicotiana benthamiana mesophyll cells. In this expression system we used AtTPK4 as a control for voltage-independent A. thaliana channels. Agrobacteria harbouring GFP-tagged constructs with the coding sequences of AKT2 and AtTPK4 were infiltrated into intact tobacco leaves. With quantitative RT-PCR analyses channel transcripts of AKT2 and AtTPK4 were determined in transformed leaves. These results were confirmed by Western blots with V5 epitope-tagged AKT2 and AtTPK4 proteins, showing that the channel protein was indeed synthesised. For functional analysis of these channels, mesophyll protoplasts were isolated from infiltrated leaf sections. Patch-clamp studies revealed that AKT2 channels in mesophyll protoplasts retained Ca2+ and pH sensitivity, characteristics of the heterologously expressed protein, but displayed pronounced differences in voltage-dependence and kinetics. AKT2-transformed mesophyll cells, displayed inward-rectifying, rather than voltage-independent K+ channels, initially characterised in AKT2-expressing animal cells. In contrast, AtTPK4 showed the same electrophysiological characteristics both, in oocytes and plant cells. Our data suggest that heterologous systems do not always possess all regulatory components for functional expression of plant channels. Therefore, transient expression of plant proteins in planta provides an additional research tool for rapid biophysical analysis of plant ion channels.
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