ArticleTransgenic research2007
The GUS reporter-aided analysis of the promoter activities of a rice metallothionein gene reveals different regulatory regions responsible for tissue-specific and inducible expression in transgenic Arabidopsis.
Article in Transgenic research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 56 citations in OpenAlex.
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- Characterization of the Metallothionein Gene Family inAntioxidants (Basel, Switzerland) · 2023Article
- Genome-Wide Analysis of the Expansin Gene Family inInternational journal of molecular sciences · 2023Article
- Changes in physio-biochemical parameters and expression of metallothioneins in Avena sativa L. in response to drought.Scientific reports · 2023Article
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- Genome-wide profiling of drought-tolerant Arabidopsis plants over-expressing chickpea MT1 gene reveals transcription factors implicated in stress modulation.Functional & integrative genomics · 2022Article
- Binding of the transcription factor MYC2-like to the ABRE of the OsCYP2 promoter enhances salt tolerance in Oryza sativa.PloS one · 2022Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2021Article
- Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response.International journal of molecular sciences · 2017Article
- Fruit preferential activity of the tomato RIP1 gene promoter in transgenic tomato and Arabidopsis.Molecular genetics and genomics : MGG · 2017Article
- Bean metal-responsive element-binding transcription factor confers cadmium resistance in tobacco.Plant physiology · 2015Article
- GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots.BMC plant biology · 2014Article
- Characterization of a maize Wip1 promoter in transgenic plants.International journal of molecular sciences · 2013Article
- Wheat chloroplast targeted sHSP26 promoter confers heat and abiotic stress inducible expression in transgenic Arabidopsis Plants.PloS one · 2013Article
- Analysis of transcriptional and upstream regulatory sequence activity of two environmental stress-inducible genes, NBS-Str1 and BLEC-Str8, of rice.Transgenic research · 2012Article
- Three Brassica rapa metallothionein genes are differentially regulated under various stress conditions.Molecular biology reports · 2012Article
- Article
- The Arabidopsis EAR-motif-containing protein RAP2.1 functions as an active transcriptional repressor to keep stress responses under tight control.BMC plant biology · 2010Article
- Heavy metal and abiotic stress inducible metallothionein isoforms from Prosopis juliflora (SW) D.C. show differences in binding to heavy metals in vitro.Molecular genetics and genomics : MGG · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To gain a better understanding of the regulatory mechanism of plant metallothionein (MT) genes, a chimeric expression unit consisting of the beta-glucuronidase (gusA) reporter gene under the control of a 1,324 bp fragment of the rice MT (ricMT) promoter was introduced into Arabidopsis via Agrobacterium tumefaciens. The strongest histochemical staining for GUS activity was observed in the cotyledons and hypocotyls of the transgenic seedlings and in the stigma, filaments and anthers of young and mature flowers, and especially in the wounded tissues of transgenic plants. In contrast, a relatively low level of reporter gene expression was seen in the young roots of transgenic seedlings and no GUS activity was detected in the stems, seeds and leaves, but GUS activity was observed in cotyledons and the first two true leaves. Promoter analysis of 5' deletions further identified several important regions responsible for organ-specific expression including roots, flowers and wound induction, light and ABA, Cu and Zn responses. These findings demonstrate that a 1,324 bp fragment of the rice MT promoter performs a complicated transcriptional regulation with clearly functional regions in a model plant, and provide an important insight into the transcriptional regulation mechanisms that operate the temporal- and spatial-specific expression and stress responses of the rice MT gene. These results suggest that the ricMT promoter and its functional regions are potentially useful in genetic engineering of plants to express the desired genes whose products are preferentially needed in roots, flowers and wound induction.
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