ArticleProceedings of the National Academy of Sciences of the United States of America1991
Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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98 citing papers in PubMed, 299 citations in OpenAlex.
- An X-linked sex determination mechanism in cannabis and hop.Nature communications · 2026Article
- 14-3-3 λ suppresses ethylene-mediated root growth inhibition through EIN3/EIL1 in Aratbidopsis.Plant physiology · 2026Article
- GWAS and haplotype analysis reveal the intricate interplay of hormones in rice seed germination under hypoxic conditions.3 Biotech · 2025Article
- An X-linked sex determination mechanism in cannabis and hop.bioRxiv : the preprint server for biology · 2025Article
- A comprehensive model of tomato fruit ripening regulation by the transcription factors NOR-like1, NAC-NOR, and MADS-RIN.Plant physiology · 2025Article
- Ethylene biosynthesis in legumes: gene identification and expression during early symbiotic stages.Journal of experimental botany · 2025Article
- Appraisal of the Role of Gaseous Signaling Molecules in Thermo-Tolerance Mechanisms in Plants.Plants (Basel, Switzerland) · 2024Review
- Discovery of candidate genes involved in ethylene biosynthesis and signal transduction pathways related to peach bud cold resistance.Frontiers in genetics · 2024Article
- Genome-Wide Identification and Expression Analysis of 1-Aminocyclopropane-1-Carboxylate Synthase (Plants (Basel, Switzerland) · 2023Article
- Activation ofPlants (Basel, Switzerland) · 2023Article
- Elevated methylglyoxal levels inhibit tomato fruit ripening by preventing ethylene biosynthesis.Plant physiology · 2023Article
- Diverse plant promoting bacterial species differentially improve tomato plant fitness under water stress.Frontiers in plant science · 2023Article
- Phylogenesis of the Functional 1-Aminocyclopropane-1-Carboxylate Oxidase of Fungi and Plants.Journal of fungi (Basel, Switzerland) · 2022Article
- Organelles and phytohormones: a network of interactions in plant stress responses.Journal of experimental botany · 2022Review
- Preparation and Application of In-Situ Loaded Silver Nanoparticles Antibacterial Fresh-Keeping Composite Paper.Polymers · 2022Article
- Potential Roles of 1-Aminocyclopropane-1-carboxylic Acid Synthase Genes in the Response ofPlants (Basel, Switzerland) · 2022Article
- TheFrontiers in plant science · 2022Article
- A 1-aminocyclopropane-1-carboxylic-acid (ACC) dipeptide elicits ethylene responses through ACC-oxidase mediated substrate promiscuity.Frontiers in plant science · 2022Article
- To Fight or to Grow: The Balancing Role of Ethylene in Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2021Review
- A novel pine wood nematode effector, BxSCD1, suppresses plant immunity and interacts with an ethylene-forming enzyme in pine.Molecular plant pathology · 2021Article
38 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ethylene-forming enzyme (EFE), which catalyzes the last step in the biosynthesis of the plant hormone ethylene, has never been purified and no molecular probes are available. Recently, a putative cDNA clone for tomato EFE (pTOM13) has been identified by inhibiting ethylene synthesis with an antisense gene expressed in transgenic plants. A direct test of its function has been made by expression of a pTOM13 gene in Saccharomyces cerevisiae. After cloning artefacts were discovered in the 5' region of the cDNA, a corrected cDNA (pRC13) was created by the fusion of the 5' end of a genomic clone to the 3' end of the cDNA and expressed in S. cerevisiae. Cultures of transformed yeast converted 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene, whereas control cells did not. This EFE activity displays similar characteristics to EFE found in plant tissue: it converts the trans isomer of the ACC analogue 1-amino-2-ethylcyclopropane-1-carboxylic acid to 1-butene in preference to the cis isomer, and it is strongly inhibited by cobaltous ions and 1,10-phenanthroline. Furthermore, information gained from the activity of effectors on yeast EFE activity supports the hypothesis that EFE is one of a group of hydroxylase enzymes.
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