ArticleProceedings of the National Academy of Sciences of the United States of America2006
Existence of an operative pathway from the endoplasmic reticulum to the immature poxvirus membrane.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- Megaviruses contain various genes encoding for eukaryotic vesicle trafficking factors.Traffic (Copenhagen, Denmark) · 2022Article
- A Deleted Deletion Site in a New Vector Strain and Exceptional Genomic Stability of Plaque-Purified Modified Vaccinia Ankara (MVA).Virologica Sinica · 2020Article
- Localization ofViruses · 2019Article
- Enigmatic origin of the poxvirus membrane from the endoplasmic reticulum shown by 3D imaging of vaccinia virus assembly mutants.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Endoplasmic Reticulum: The Favorite Intracellular Niche for Viral Replication and Assembly.Viruses · 2016Review
- Poxviruses Encode a Reticulon-Like Protein that Promotes Membrane Curvature.Cell reports · 2016Article
- Characterization of a large, proteolytically processed cowpox virus membrane glycoprotein conserved in most chordopoxviruses.Virology · 2015Article
- Poxvirus membrane biogenesis.Virology · 2015Review
- Review
- Direct formation of vaccinia virus membranes from the endoplasmic reticulum in the absence of the newly characterized L2-interacting protein A30.5.Journal of virology · 2013Article
- Association of the vaccinia virus A11 protein with the endoplasmic reticulum and crescent precursors of immature virions.Journal of virology · 2013Article
- Analysis of viral membranes formed in cells infected by a vaccinia virus L2-deletion mutant suggests their origin from the endoplasmic reticulum.Journal of virology · 2013Article
- Membrane assembly during the infection cycle of the giant Mimivirus.PLoS pathogens · 2013Article
- Biogenesis of the vaccinia virus membrane: genetic and ultrastructural analysis of the contributions of the A14 and A17 proteins.Journal of virology · 2013Article
- Vaccinia virus virion membrane biogenesis protein A11 associates with viral membranes in a manner that requires the expression of another membrane biogenesis protein, A6.Journal of virology · 2012Article
- Vaccinia virus A6 is essential for virion membrane biogenesis and localization of virion membrane proteins to sites of virion assembly.Journal of virology · 2012Article
- Mechanism of collapse of endoplasmic reticulum cisternae during African swine fever virus infection.Traffic (Copenhagen, Denmark) · 2012Article
- Vaccinia virus L2 protein associates with the endoplasmic reticulum near the growing edge of crescent precursors of immature virions and stabilizes a subset of viral membrane proteins.Journal of virology · 2011Article
- Participation of vaccinia virus l2 protein in the formation of crescent membranes and immature virions.Journal of virology · 2011Article
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
In thin sections of cells infected with vaccinia virus or other poxviruses, the viral membrane is first discerned as a crescent or circle lacking obvious continuity with a cellular organelle, presenting an appearance of de novo membrane biogenesis. This notion, which many consider heretical, is nevertheless consistent with the absence of a signature of endoplasmic reticulum (ER) trafficking, such as signal peptide cleavage or glycosylation, in any of the numerous viral membrane proteins. The purpose of this study was to determine whether an operative pathway exists between the ER and the immature virion membrane. We showed that the highly conserved A9 viral membrane protein was inserted into the ER of uninfected cells with the same topology as in viral membranes. Next, we found that replacement of the nonessential cytoplasmic tail of A9 with one containing COPII-binding sites reduced incorporation of the modified A9 into viral membranes and led to its accumulation in the Golgi apparatus, implying that A9 was inserted into the ER and then diverted from its natural path. Most importantly, we demonstrated cleavage of a heterologous signal peptide fused to the N-terminal region of A9 and localized the truncated protein in immature and mature virions. Additionally, immunoelectron micrographs showed A9 in tubules containing protein disulfide isomerase, an ER lumenal protein, near immature viral membranes. The present data provide strong evidence for an operative pathway from ER domains within the virus factory to the viral membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.