ArticleThe Journal of cell biology2006
A conserved late endosome-targeting signal required for Doa4 deubiquitylating enzyme function.
Article in The Journal of cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Post-Golgi Trafficking in Plant Cells.Sub-cellular biochemistry · 2026Review
- A Saccharomyces cerevisiae knockout screen for genes critical for growth under sulfur- and nitrogen-limited conditions reveals intracellular sorting via vesicular transport systems.G3 (Bethesda, Md.) · 2025Article
- Illuminating the Cryptococcus neoformans species complex: unveiling intracellular structures with fluorescent-protein-based markers.Genetics · 2024Article
- Sulfuriroseicoccus oceanibius gen. nov., sp. nov., a representative of the phylum Verrucomicrobia with a special cytoplasmic membrane.Antonie van Leeuwenhoek · 2022Article
- The structure and function of deubiquitinases: lessons from budding yeast.Open biology · 2020Review
- Genetic Selection Based on a Ste6G3 (Bethesda, Md.) · 2020Article
- The degradation-promoting roles of deubiquitinases Ubp6 and Ubp3 in cytosolic and ER protein quality control.PloS one · 2020Article
- A balance of deubiquitinating enzymes controls cell cycle entry.Molecular biology of the cell · 2018Article
- Multiplexed, Proteome-Wide Protein Expression Profiling: Yeast Deubiquitylating Enzyme Knockout Strains.Journal of proteome research · 2015Article
- The ESCRT-III adaptor protein Bro1 controls functions of regulator for free ubiquitin chains 1 (Rfu1) in ubiquitin homeostasis.The Journal of biological chemistry · 2014Article
- Ubiquitin-dependent sorting in endocytosis.Cold Spring Harbor perspectives in biology · 2014Review
- The yeast Alix homolog Bro1 functions as a ubiquitin receptor for protein sorting into multivesicular endosomes.Developmental cell · 2013Article
- Doa4 function in ILV budding is restricted through its interaction with the Vps20 subunit of ESCRT-III.Journal of cell science · 2013Article
- Environmental stresses disrupt telomere length homeostasis.PLoS genetics · 2013Article
- The ubiquitin-proteasome system of Saccharomyces cerevisiae.Genetics · 2012Review
- How ubiquitin functions with ESCRTs.Traffic (Copenhagen, Denmark) · 2011Review
- Structural basis for ESCRT-III CHMP3 recruitment of AMSH.Structure (London, England : 1993) · 2011Article
- MVB vesicle formation: ESCRT-dependent, ESCRT-independent and everything in between.Current opinion in cell biology · 2011Review
- Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus.Journal of virology · 2011Article
- Molecular mechanisms of ubiquitin-dependent membrane traffic.Annual review of biophysics · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Enzyme specificity in vivo is often controlled by subcellular localization. Yeast Doa4, a deubiquitylating enzyme (DUB), removes ubiquitin from membrane proteins destined for vacuolar degradation. Doa4 is recruited to the late endosome after ESCRT-III (endosomal sorting complex required for transport III) has assembled there. We show that an N-terminal segment of Doa4 is sufficient for endosome association. This domain bears four conserved elements (boxes A-D). Deletion of the most conserved of these, A or B, prevents Doa4 endosomal localization. These mutants cannot sustain ubiquitin-dependent proteolysis even though neither motif is essential for deubiquitylating activity. Ubiquitin-specific processing protease 5 (Ubp5), the closest paralogue of Doa4, has no functional overlap. Ubp5 concentrates at the bud neck; its N-terminal domain is critical for this. Importantly, substitution of the Ubp5 N-terminal domain with that of Doa4 relocalizes the Ubp5 enzyme to endosomes and provides Doa4 function. This is the first demonstration of a physiologically important DUB subcellular localization signal and provides a striking example of the functional diversification of DUB paralogues by the evolution of alternative spatial signals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.