ArticleMolecular and cellular biology2007
Analysis of sequence and structural features that identify the B/C motif of U3 small nucleolar RNA as the recognition site for the Snu13p-Rrp9p protein pair.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Small but Mighty-The Emerging Role of snoRNAs in Hematological Malignancies.Non-coding RNA · 2021Review
- Emerging Data on the Diversity of Molecular Mechanisms Involving C/D snoRNAs.Non-coding RNA · 2021Review
- Synergistic defects in pre-rRNA processing from mutations in the U3-specific protein Rrp9 and U3 snoRNA.Nucleic acids research · 2020Article
- Gene expression inPeerJ · 2019Article
- Mapping targets for small nucleolar RNAs in yeast.Wellcome open research · 2018Article
- Implication of the box C/D snoRNP assembly factor Rsa1p in U3 snoRNP assembly.Nucleic acids research · 2017Article
- Article
- Small Non-coding RNA Abundance in Adrenocortical Carcinoma: A Footprint of a Rare Cancer.Journal of genomics · 2017Article
- RNA-Seq Validation of RNAi Identifies Additional Gene Connectivity in Tribolium castaneum (Coleoptera: Tenebrionidae).Journal of insect science (Online) · 2017Article
- Integrative structural analysis of the UTPB complex, an early assembly factor for eukaryotic small ribosomal subunits.Nucleic acids research · 2016Article
- Characterization of the interaction between protein Snu13p/15.5K and the Rsa1p/NUFIP factor and demonstration of its functional importance for snoRNP assembly.Nucleic acids research · 2014Article
- Post-transcriptional regulation of myotube elongation and myogenesis by Hoi Polloi.Development (Cambridge, England) · 2013Article
- Structural and functional analysis of the U3 snoRNA binding protein Rrp9.RNA (New York, N.Y.) · 2013Article
- A second base pair interaction between U3 small nucleolar RNA and the 5'-ETS region is required for early cleavage of the yeast pre-ribosomal RNA.Nucleic acids research · 2011Article
- Human box C/D snoRNAs with miRNA like functions: expanding the range of regulatory RNAs.Nucleic acids research · 2011Article
- YAO is a nucleolar WD40-repeat protein critical for embryogenesis and gametogenesis in Arabidopsis.BMC plant biology · 2010Article
- U3 snoRNA genes are multi-copy and frequently linked to U5 snRNA genes in Euglena gracilis.BMC genomics · 2009Article
- Non-coding RNA annotation of the genome of Trichoplax adhaerens.Nucleic acids research · 2009Article
- An improved definition of the RNA-binding specificity of SECIS-binding protein 2, an essential component of the selenocysteine incorporation machinery.Nucleic acids research · 2007Article
- The small subunit processome in ribosome biogenesis—progress and prospects.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The eukaryal Snu13p/15.5K protein binds K-turn motifs in U4 snRNA and snoRNAs. Two Snu13p/15.5K molecules bind the nucleolar U3 snoRNA required for the early steps of preribosomal processing. Binding of one molecule on the C'/D motif allows association of proteins Nop1p, Nop56p, and Nop58p, whereas binding of the second molecule on the B/C motif allows Rrp9p recruitment. To understand how the Snu13p-Rrp9p pair recognizes the B/C motif, we first improved the identification of RNA determinants required for Snu13p binding by experiments using the systematic evolution of ligands by exponential enrichment. This demonstrated the importance of a U.U pair stacked on the sheared pairs and revealed a direct link between Snu13p affinity and the stability of helices I and II. Sequence and structure requirements for efficient association of Rrp9p on the B/C motif were studied in yeast cells by expression of variant U3 snoRNAs and immunoselection assays. A G-C pair in stem II, a G residue at position 1 in the bulge, and a short stem I were found to be required. The data identify the in vivo function of most of the conserved residues of the U3 snoRNA B/C motif. They bring important information to understand how different K-turn motifs can recruit different sets of proteins after Snu13p association.
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