ArticleMolecular and cellular biology2007
Puralpha and Purbeta collaborate with Sp3 to negatively regulate beta-myosin heavy chain gene expression during skeletal muscle inactivity.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Purine-rich element binding protein alpha: a DNA/RNA binding protein with multiple roles in cancers.Molecular medicine (Cambridge, Mass.) · 2025Review
- Aromatic Residues Dictate the Transcriptional Repressor and Single-Stranded DNA Binding Activities of Purine-Rich Element Binding Protein B.Biochemistry · 2023Article
- Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes.International journal of molecular sciences · 2023Article
- MicroRNAs as the Sentinels of Redox and Hypertrophic Signalling.International journal of molecular sciences · 2022Review
- The Role of GSK-3β in the Regulation of Protein Turnover, Myosin Phenotype, and Oxidative Capacity in Skeletal Muscle under Disuse Conditions.International journal of molecular sciences · 2021Review
- The Molecular Function of PURA and Its Implications in Neurological Diseases.Frontiers in genetics · 2021Review
- circSamd4 represses myogenic transcriptional activity of PUR proteins.Nucleic acids research · 2020Article
- Deficiency ofFrontiers in sports and active living · 2019Article
- Vestigial-like 2 contributes to normal muscle fiber type distribution in mice.Scientific reports · 2017Article
- Coupling of mitochondrial function and skeletal muscle fiber type by a miR-499/Fnip1/AMPK circuit.EMBO molecular medicine · 2016Article
- Electrical stimulation influences chronic intermittent hypoxia-hypercapnia induction of muscle fibre transformation by regulating the microRNA/Sox6 pathway.Scientific reports · 2016Article
- Exercise training in hypertension: Role of microRNAs.World journal of cardiology · 2014Review
- Structural basis of multisite single-stranded DNA recognition and ACTA2 repression by purine-rich element binding protein B (Purβ).Biochemistry · 2013Article
- Nuclear receptor/microRNA circuitry links muscle fiber type to energy metabolism.The Journal of clinical investigation · 2013Article
- Targeted deletion of microRNA-22 promotes stress-induced cardiac dilation and contractile dysfunction.Circulation · 2012Article
- The MyomiR network in skeletal muscle plasticity.Exercise and sport sciences reviews · 2011Review
- Feeding blueberry diets in early life prevent senescence of osteoblasts and bone loss in ovariectomized adult female rats.PloS one · 2011Article
- Isolation and characterization of the core single-stranded DNA-binding domain of purine-rich element binding protein B (Purβ).Biochemical and biophysical research communications · 2010Article
- A bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Pur alpha).The Journal of biological chemistry · 2009Article
- Evidence of MyomiR network regulation of beta-myosin heavy chain gene expression during skeletal muscle atrophy.Physiological genomics · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Adult skeletal muscle retains the capability of transcriptional reprogramming. This attribute is readily observable in the non-weight-bearing (NWB) soleus muscle, which undergoes a slow-to-fast fiber type transition concurrent with decreased beta-myosin heavy chain (betaMyHC) gene expression. Our previous work showed that Sp3 contributes to decreased betaMyHC gene expression under NWB conditions. In this study, we demonstrate that physical and functional interactions between Sp3, Puralpha, and Purbeta proteins mediate repression of betaMyHC expression under NWB conditions. Binding of Puralpha or Purbeta to the single-stranded betaMyHC distal negative regulatory element-sense strand (dbetaNRE-S) element is markedly increased under NWB conditions. Ectopic expression of Puralpha and Purbeta decreased betaMyHC reporter gene expression, while mutation of the dbetaNRE-S element increased expression in C2C12 myotubes. The dbetaNRE-S element conferred Pur-dependent decreased expression on a minimal thymidine kinase promoter. Short interfering RNA sequences specific for Sp3 or for Puralpha and Purbeta decreased endogenous Sp3 and Pur protein levels and increased betaMyHC reporter gene expression in C2C12 myotubes. Immunoprecipitation assays revealed an association between endogenous Puralpha, Purbeta, and Sp3, while chromatin immunoprecipitation assays demonstrated Puralpha, Purbeta, and Sp3 binding to the betaMyHC proximal promoter region harboring the dbetaNRE-S and C-rich elements in vivo. These data demonstrate that Pur proteins collaborate with Sp3 to regulate a transcriptional program that enables muscle cells to remodel their phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.