ArticleMolecular genetics and genomics : MGG2007
A genetic screen supports a broad role for the Drosophila insulator proteins BEAF-32A and BEAF-32B in maintaining patterns of gene expression.
Article in Molecular genetics and genomics : MGG, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- The Drosophila BEAF insulator protein interacts with the polybromo subunit of the PBAP chromatin remodeling complex.G3 (Bethesda, Md.) · 2022Article
- Overlapping but Distinct Sequences Play Roles in the Insulator and Promoter Activities of theGenetics · 2020Article
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- Insulator speckles associated with long-distance chromatin contacts.Biology open · 2016Article
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- Surviving an identity crisis: a revised view of chromatin insulators in the genomics era.Biochimica et biophysica acta · 2014Review
- A functional insulator screen identifies NURF and dREAM components to be required for enhancer-blocking.PloS one · 2014Article
- The BEAF insulator regulates genes involved in cell polarity and neoplastic growth.Developmental biology · 2012Article
- Lack of the Drosophila BEAF insulator proteins alters regulation of genes in the Antennapedia complex.Molecular genetics and genomics : MGG · 2011Article
- Chromatin insulators: a role in nuclear organization and gene expression.Advances in cancer research · 2011Review
- Chromatin insulators: lessons from the fly.Briefings in functional genomics & proteomics · 2009Review
- Genome-wide mapping of boundary element-associated factor (BEAF) binding sites in Drosophila melanogaster links BEAF to transcription.Molecular and cellular biology · 2009Article
- BEAF regulates cell-cycle genes through the controlled deposition of H3K9 methylation marks into its conserved dual-core binding sites.PLoS biology · 2008Article
- The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning.The EMBO journal · 2007Article
- Characterization of BEAF mutations isolated by homologous recombination in Drosophila.Genetics · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin domain insulators are thought to insulate adjacent genes, including their regulatory elements, from each other by organizing chromatin into functionally independent domains. Thus insulators should play a global role in gene regulation by keeping regulatory domains separated. However, this has never been demonstrated. We previously designed and characterized a transgene that is under GAL4 UAS control and encodes a dominant-negative form of the Boundary Element-Associated Factors BEAF-32A and BEAF-32B. The BID transgene encodes the BEAF self-interaction domain but lacks a DNA binding domain. Expression of BID in eye imaginal discs leads to a rough eye phenotype. Here we screen for dominant mutations that modify this eye phenotype. This assay provides evidence for cross-talk between different classes of insulators, and for a broad role of the BEAF proteins in maintaining patterns of gene expression during eye development. Most identified genes encode other insulator binding proteins, transcription factors involved in head development, or general transcription factors. Because it is unlikely that insulator function is limited to eye development, the present results support the hypothesis that insulators play a widespread role in maintaining global transcription programs.
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