ArticlePlant molecular biology2007
A combined strategy of "in silico" transcriptome analysis and web search engine optimization allows an agile identification of reference genes suitable for normalization in gene expression studies.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed.
- Transcriptome-Based Identification of Reference Genes for Expression Analysis in Cassava UnderPlants (Basel, Switzerland) · 2025Article
- Expression stability of internal reference gene in response to Trichoderma polysporum infection in Avena fatua L.Current genetics · 2021Article
- Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries.Plant methods · 2021Article
- Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley.International journal of molecular sciences · 2020Article
- Identifying optimal reference genes for the normalization of microRNA expression in cucumber under viral stress.PloS one · 2018Article
- Using RT-qPCR, Proteomics, and Microscopy to Unravel the Spatio-Temporal Expression and Subcellular Localization of Hordoindolines Across Development in Barley Endosperm.Frontiers in plant science · 2018Article
- Selection of relatively exact reference genes for gene expression studies in goosegrass (Eleusine indica) under herbicide stress.Scientific reports · 2017Article
- Article
- Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).PloS one · 2016Article
- Validation of Reference Genes for Relative Quantitative Gene Expression Studies in Cassava (Manihot esculenta Crantz) by Using Quantitative Real-Time PCR.Frontiers in plant science · 2016Article
- Tracking the best reference genes for RT-qPCR data normalization in filamentous fungi.BMC genomics · 2015Article
- Article
- Identification and validation of suitable reference genes for RT-qPCR analysis in mouse testis development.Molecular genetics and genomics : MGG · 2014Article
- Comparative analysis of gene expression among species of different ploidy.Molecular biology reports · 2014Article
- Members of the barley NAC transcription factor gene family show differential co-regulation with senescence-associated genes during senescence of flag leaves.Journal of experimental botany · 2014Article
- Validation of reference genes aiming accurate normalization of qRT-PCR data in Dendrocalamus latiflorus Munro.PloS one · 2014Article
- The choice of reference gene set for assessing gene expression in barley (Hordeum vulgare L.) under low temperature and drought stress.Molecular genetics and genomics : MGG · 2013Article
- Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition.PloS one · 2013Article
- Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.PloS one · 2013Article
- Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.Molecular genetics and genomics : MGG · 2012Article
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6 authors.
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Abstract
Traditionally housekeeping genes have been employed as endogenous reference (internal control) genes for normalization in gene expression studies. Since the utilization of single housekeepers cannot assure an unbiased result, new normalization methods involving multiple housekeeping genes and normalizing using their mean expression have been recently proposed. Moreover, since a gold standard gene suitable for every experimental condition does not exist, it is also necessary to validate the expression stability of every putative control gene on the specific requirements of the planned experiment. As a consequence, finding a good set of reference genes is for sure a non-trivial problem requiring quite a lot of lab-based experimental testing. In this work we identified novel candidate barley reference genes suitable for normalization in gene expression studies. An advanced web search approach aimed to collect, from publicly available web resources, the most interesting information regarding the expression profiling of candidate housekeepers on a specific experimental basis has been set up and applied, as an example, on stress conditions. A complementary lab-based analysis has been carried out to verify the expression profile of the selected genes in different tissues and during heat shock response. This combined dry/wet approach can be applied to any species and physiological condition of interest and can be considered very helpful to identify putative reference genes to be shortlisted every time a new experimental design has to be set up.
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