ArticleEukaryotic cell2007
Prenylation of Saccharomyces cerevisiae Chs4p Affects Chitin Synthase III activity and chitin chain length.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- How post-translational modifications in pathogenic fungi inform pathogenesis and immune responses.PLoS pathogens · 2026Article
- The chitin synthase regulator CSR-3 promotes cellular integrity during cell-cell fusion in the filamentous ascomycete fungus Neurospora crassa.PLoS genetics · 2025Article
- Transient septin sumoylation steers a Fir1-Skt5 protein complex between the split septin ring.The Journal of cell biology · 2024Article
- Role of paracoccin onVirulence · 2023Article
- Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation.Applied microbiology and biotechnology · 2022Article
- Chitin Synthesis in Yeast: A Matter of Trafficking.International journal of molecular sciences · 2022Review
- Structural basis for inhibition and regulation of a chitin synthase from Candida albicans.Nature structural & molecular biology · 2022Article
- Priming and elongation of chitin chains: Implications for chitin synthase mechanism.Cell surface (Amsterdam, Netherlands) · 2019Article
- Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory.Microbial cell factories · 2019Article
- Review
- QTL mapping of the production of wine aroma compounds by yeast.BMC genomics · 2012Article
- Regulation of expression, activity and localization of fungal chitin synthases.Medical mycology · 2012Review
- The FN3 and BRCT motifs in the exomer component Chs5p define a conserved module that is necessary and sufficient for its function.Cellular and molecular life sciences : CMLS · 2011Article
- A novel role of the yeast CaaX protease Ste24 in chitin synthesis.Molecular biology of the cell · 2010Article
- Molecular characterization of the cis-prenyltransferase of Giardia lamblia.Glycobiology · 2010Article
- The fission yeast SEL1 domain protein Cfh3p: a novel regulator of the glucan synthase Bgs1p whose function is more relevant under stress conditions.The Journal of biological chemistry · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Chs4p (Cal2/Csd4/Skt5) was identified as a protein factor physically interacting with Chs3p, the catalytic subunit of chitin synthase III (CSIII), and is indispensable for its enzymatic activity in vivo. Chs4p contains a putative farnesyl attachment site at the C-terminal end (CVIM motif) conserved in Chs4p of Saccharomyces cerevisiae and other fungi. Several previous reports questioned the role of Chs4p prenylation in chitin biosynthesis. In this study we reinvestigated the function of Chs4p prenylation. We provide evidence that Chs4p is farnesylated by showing that purified Chs4p is recognized by anti-farnesyl antibody and is a substrate for farnesyl transferase (FTase) in vitro and that inactivation of FTase increases the amount of unmodified Chs4p in yeast cells. We demonstrate that abolition of Chs4p prenylation causes a approximately 60% decrease in CSIII activity, which is correlated with a approximately 30% decrease in chitin content and with increased resistance to the chitin binding compound calcofluor white. Furthermore, we show that lack of Chs4p prenylation decreases the average chain length of the chitin polymer. Prenylation of Chs4p, however, is not a factor that mediates plasma membrane association of the protein. Our results provide evidence that the prenyl moiety attached to Chs4p is a factor modulating the activity of CSIII both in vivo and in vitro.
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