ArticleJournal of bacteriology2007
Similarity and differences in the Lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 107 citations in OpenAlex.
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- Comparative analysis of Lactobacillus gasseri from Chinese subjects reveals a new species-level taxa.BMC genomics · 2020Article
- Genome-based species-specific primers for rapid identification of six species of Lactobacillus acidophilus group using multiplex PCR.PloS one · 2020Article
- Complete Genome Sequencing and Comparative Genome Characterization ofFrontiers in genetics · 2019Article
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- Identification of a mouse Lactobacillus johnsonii strain with deconjugase activity against the FXR antagonist T-β-MCA.PloS one · 2017Article
- EpsA is an essential gene in exopolysaccharide production in Lactobacillus johnsonii FI9785.Microbial biotechnology · 2016Article
- Strain-Specific Features of Extracellular Polysaccharides and Their Impact on Lactobacillus plantarum-Host Interactions.Applied and environmental microbiology · 2016Article
- Functional Analysis of an S-Layer-Associated Fibronectin-Binding Protein in Lactobacillus acidophilus NCFM.Applied and environmental microbiology · 2016Article
- Conserved S-Layer-Associated Proteins Revealed by Exoproteomic Survey of S-Layer-Forming Lactobacilli.Applied and environmental microbiology · 2016Article
- Exopolysaccharide production and ropy phenotype are determined by two gene clusters in putative probiotic strain Lactobacillus paraplantarum BGCG11.Applied and environmental microbiology · 2015Article
- Comparative genomics of Lactobacillus crispatus suggests novel mechanisms for the competitive exclusion of Gardnerella vaginalis.BMC genomics · 2014Article
- The domestication of the probiotic bacterium Lactobacillus acidophilus.Scientific reports · 2014Article
- Unusual genome complexity in Lactobacillus salivarius JCM1046.BMC genomics · 2014Article
- H(2)O(2) production in species of the Lactobacillus acidophilus group: a central role for a novel NADH-dependent flavin reductase.Applied and environmental microbiology · 2014Article
- Genomic overview and biological functions of exopolysaccharide biosynthesis in Bifidobacterium spp.Applied and environmental microbiology · 2014Review
- Development of a tiered multilocus sequence typing scheme for members of the Lactobacillus acidophilus complex.Applied and environmental microbiology · 2013Article
- Exploration of the genomic diversity and core genome of the Bifidobacterium adolescentis phylogenetic group by means of a polyphasic approach.Applied and environmental microbiology · 2013Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A set of lactobacilli were investigated by polyphasic analysis. Multilocus sequence analysis, DNA typing, microarray analysis, and in silico whole-genome alignments provided a remarkably consistent pattern of similarity within the Lactobacillus acidophilus complex. On microarray analysis, 17 and 5% of the genes from Lactobacillus johnsonii strain NCC533 represented variable and strain-specific genes, respectively, when tested against four independent isolates of L. johnsonii. When projected on the NCC533 genome map, about 10 large clusters of variable genes were identified, and they were enriched around the terminus of replication. A quarter of the variable genes and two-thirds of the strain-specific genes were associated with mobile DNA. Signatures for horizontal gene transfer and modular evolution were found in prophages and in DNA from the exopolysaccharide biosynthesis cluster. On microarray hybridizations, Lactobacillus gasseri strains showed a shift to significantly lower fluorescence intensities than the L. johnsonii test strains, and only genes encoding very conserved cellular functions from L. acidophilus hybridized to the L. johnsonii array. In-silico comparative genomics showed extensive protein sequence similarity and genome synteny of L. johnsonii with L. gasseri, L. acidophilus, and Lactobacillus delbrueckii; moderate synteny with Lactobacillus casei; and scattered X-type sharing of protein sequence identity with the other sequenced lactobacilli. The observation of a stepwise decrease in similarity between the members of the L. acidophilus group suggests a strong element of vertical evolution in a natural phylogenetic group. Modern whole-genome-based techniques are thus a useful adjunct to the clarification of taxonomical relationships in problematic bacterial groups.
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