ArticleJournal of bacteriology2007
Expression of the major porin gene mspA is regulated in Mycobacterium smegmatis.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- The mycomembrane proteins PorH and ProtX are inserted at polar growth zones and linked to the cell wall.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The conserved translation factor LepA is required for optimal synthesis of a porin family inJournal of bacteriology · 2020Article
- Sporulation, bacterial cell envelopes and the origin of life.Nature reviews. Microbiology · 2016Review
- Inverse correlation between salt tolerance and host-adaptation in mycobacteria.BMC research notes · 2016Article
- Hetero-oligomeric MspA pores in Mycobacterium smegmatis.FEMS microbiology letters · 2016Article
- Draft Genome Sequence of the Bacterium Gordonia jacobaea, a New Member of the Gordonia Genus.Genome announcements · 2015Article
- The rv1184c locus encodes Chp2, an acyltransferase in Mycobacterium tuberculosis polyacyltrehalose lipid biosynthesis.Journal of bacteriology · 2015Article
- Gene replacement in Mycobacterium chelonae: application to the construction of porin knock-out mutants.PloS one · 2014Article
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- Expression of the ompATb operon accelerates ammonia secretion and adaptation of Mycobacterium tuberculosis to acidic environments.Molecular microbiology · 2011Article
- Regulation of expression of the region 3 promoter of the Escherichia coli K5 capsule gene cluster involves H-NS, SlyA, and a large 5' untranslated region.Journal of bacteriology · 2009Article
- Characterisation of porin genes from Mycobacterium fortuitum and their impact on growth.BMC microbiology · 2009Article
- Identification of a novel multidrug efflux pump of Mycobacterium tuberculosis.Antimicrobial agents and chemotherapy · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MspA is the major porin of Mycobacterium smegmatis and is important for diffusion of small and hydrophilic solutes across its unique outer membrane. The start point of transcription of the mspA gene was mapped by primer extension and S1 nuclease experiments. The main promoter driving transcription of mspA was identified by single point mutations in lacZ fusions and resembled sigma(A) promoters of M. smegmatis. However, a 500-bp upstream fragment including P(mspA) in a transcriptional fusion with lacZ yielded only low beta-galactosidase activity, whereas activity increased 12-fold with a 700-bp fragment. Activation of P(mspA) by the 200-bp element was almost eliminated by increasing the distance by 14 bp, indicating binding of an activator protein. The chromosomal mspA transcript had a size of 900 bases and was very stable with a half-life of 6 minutes, whereas the stabilities of episomal mspA transcripts with three other 5' untranslated region (UTRs) were three- to sixfold reduced, indicating a stabilizing role of the native 5' UTR of mspA. Northern blot experiments revealed that the amount of mspA mRNA was increased under nitrogen limitation but reduced under carbon and phosphate limitation at 42 degrees C in stationary phase in the presence of 0.5 M sodium chloride, 18 mM hydrogen peroxide, and 10% ethanol and at acidic pH. These results show for the first time that M. smegmatis regulates porin gene expression to optimize uptake of certain nutrients and to protect itself from toxic solutes.
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