ArticleApplied and environmental microbiology2007
Multiplex quantitative real-time reverse transcriptase PCR for F+-specific RNA coliphages: a method for use in microbial source tracking.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 81 citations in OpenAlex.
- A multi-tiered approach to assess fecal pollution in an urban watershed: Bacterial and viral indicators and sediment microbial communities.The Science of the total environment · 2024Article
- Occurrence of Staphylococcus spp. and investigation of fecal and animal viral contaminations in livestock, river water, and sewage from Tunisia and Romania.Environmental monitoring and assessment · 2020Article
- Preliminary Source Tracking of Male-Specific (FFood and environmental virology · 2017Article
- Distributions of Fecal Markers in Wastewater from Different Climatic Zones for Human Fecal Pollution Tracking in Australian Surface Waters.Applied and environmental microbiology · 2016Article
- Persistence of F-Specific RNA Coliphages in Surface Waters from a Produce Production Region along the Central Coast of California.PloS one · 2016Article
- Application of F⁺RNA Coliphages as Source Tracking Enteric Viruses on Parsley and Leek Using RT-PCR.Food and environmental virology · 2015Article
- U.S. Recreational Water Quality Criteria: A Vision for the Future.International journal of environmental research and public health · 2015Article
- Enzyme treatment reverse transcription-PCR to differentiate infectious and inactivated F-specific RNA phages.Applied and environmental microbiology · 2014Article
- Coherence among different microbial source tracking markers in a small agricultural stream with or without livestock exclusion practices.Applied and environmental microbiology · 2013Article
- Molecular Approach to Microbiological Examination of Water Quality in the Grand Bay National Estuarine Research Reserve (NERR) in Mississippi, USA.Environmental health insights · 2013Article
- Effects of ionic strength on bacteriophage MS2 behavior and their implications for the assessment of virus retention by ultrafiltration membranes.Applied and environmental microbiology · 2011Article
- Occurrence, survival, and persistence of human adenoviruses and F-specific RNA phages in raw groundwater.Applied and environmental microbiology · 2010Article
- Performance assessment PCR-based assays targeting bacteroidales genetic markers of bovine fecal pollution.Applied and environmental microbiology · 2010Article
- Molecular characterization of bacteriophages for microbial source tracking in Korea.Applied and environmental microbiology · 2009Article
- Escherichia coli populations in Great Lakes waterfowl exhibit spatial stability and temporal shifting.Applied and environmental microbiology · 2009Article
- Application of PCR-based methods to assess the infectivity of enteric viruses in environmental samples.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is well documented that microbial contamination of coastal waters poses a significant risk to human health through recreational exposure and consumption of shellfish. Identifying the source of microbial contamination (microbial source tracking) plays a dominant role in enabling effective management and remediation strategies. One method used to determine the source of the contamination is quantification of the ratio of the four subgroups of F+-specific RNA coliphages (family Leviviridae) in impacted water samples. Because of typically low concentrations in the environment, enrichment assays are performed prior to detection, even though differential replication rates have been reported. These assays are also compromised by differential loss of phage infectivity among subgroups after release into the environment, thus obscuring the initial ratio. Here, a culture-independent multiplex real-time reverse transcriptase-PCR (RT-PCR) protocol for the simultaneous quantification of all four subgroups of F+-specific RNA coliphages using novel primer sets and molecular beacons is presented. This assay is extremely sensitive, achieving detection with as few as 10 copies of isolated coliphage RNA, and is linear for a minimum of six orders of magnitude. During survival experiments, the real-time RT-PCR technique was able to quantify coliphages in seawater when culture-based double agar layer assay failed. While infectivity was lost at different rates at the subgroup level, decay constants in seawater, calculated using the real-time RT-PCR estimates, did not vary among subgroups. The accurate determination of the in situ concentration of F+-specific RNA coliphages using this method will facilitate more effective remediation strategies for impacted environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.