ArticleApplied and environmental microbiology2007
Novel quantitative biosystem for modeling physiological fluid shear stress on cells.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 72 citations in OpenAlex.
- Article
- Microbiology of human spaceflight: microbial responses to mechanical forces that impact health and habitat sustainability.Microbiology and molecular biology reviews : MMBR · 2024Review
- Article
- Bacterial Virulence and Prevention for Human Spaceflight.Life (Basel, Switzerland) · 2023Article
- Article
- CAMDLES: CFD-DEM Simulation of Microbial Communities in Spaceflight and Artificial Microgravity.Life (Basel, Switzerland) · 2022Article
- Spaceflight Analogue Culture Enhances the Host-Pathogen Interaction BetweenFrontiers in cellular and infection microbiology · 2022Article
- A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions.Microorganisms · 2021Review
- The influence of spaceflight and simulated microgravity on bacterial motility and chemotaxis.NPJ microgravity · 2021Review
- Bactericidal efficiency of micro- and nanostructured surfaces: a critical perspective.RSC advances · 2021Review
- Human Placental Trophoblasts Are Resistant toFrontiers in microbiology · 2021Article
- Efficacy of A Poly(MeOEGMA) Brush on the Prevention ofAntibiotics (Basel, Switzerland) · 2020Article
- Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age.Infection and immunity · 2018Review
- An integrated biomanufacturing platform for the large-scale expansion and neuronal differentiation of human pluripotent stem cell-derived neural progenitor cells.Acta biomaterialia · 2018Article
- RhoGTPase stimulation is associated with strontium chloride treatment to counter simulated microgravity-induced changes in multipotent cell commitment.NPJ microgravity · 2017Article
- Microgravity as a biological tool to examine host-pathogen interactions and to guide development of therapeutics and preventatives that target pathogenic bacteria.Pathogens and disease · 2016Review
- Article
- A three-dimensional culture system recapitulates placental syncytiotrophoblast development and microbial resistance.Science advances · 2016Article
- Physiological fluid shear alters the virulence potential of invasive multidrug-resistant non-typhoidalNPJ microgravity · 2016Article
- The shear stress of host cell invasion: exploring the role of biomolecular complexes.PLoS pathogens · 2015Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The response of microbes to changes in the mechanical force of fluid shear has important implications for pathogens, which experience wide fluctuations in fluid shear in vivo during infection. However, the majority of studies have not cultured microbes under physiological fluid shear conditions within a range commonly encountered by microbes during host-pathogen interactions. Here we describe a convenient batch culture biosystem in which (i) the levels of fluid shear force can be varied within physiologically relevant ranges and quantified via mathematical models and (ii) large numbers of cells can be planktonically grown and harvested to examine the effect of fluid shear levels on microbial genomic and phenotypic responses. A quantitative model based on numerical simulations and in situ imaging analysis was developed to calculate the fluid shear imparted by spherical beads of different sizes on bacterial cell cultures grown in a rotating wall vessel (RWV) bioreactor. To demonstrate the application of this model, we subjected cultures of the bacterial pathogen Salmonella enterica serovar Typhimurium to three physiologically-relevant fluid shear ranges during growth in the RVW and demonstrated a progressive relationship between the applied fluid shear and the bacterial genetic and phenotypic responses. By applying this model to different cell types, including other bacterial pathogens, entire classes of genes and proteins involved in cellular interactions may be discovered that have not previously been identified during growth under conventional culture conditions, leading to new targets for vaccine and therapeutic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.