ArticleBiophysical journal2007
Cytoplasmic domain of human myelin protein zero likely folded as beta-structure in compact myelin.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- On the synergy between myelin proteins P0, MBP, and P2 in peripheral nerve major dense line formation.The FEBS journal · 2025Article
- Features of Remyelination after Transplantation of Olfactory Ensheathing Cells with Neurotrophic Factors into Spinal Cord Cysts.Bulletin of experimental biology and medicine · 2024Article
- The intrinsically disordered protein glue of the myelin major dense line: Linking AlphaFold2 predictions to experimental data.Biochemistry and biophysics reports · 2023Article
- The Epigenetic Dimension of Protein Structure Is an Intrinsic Weakness of the AlphaFold Program.Biomolecules · 2022Article
- How Does Protein Zero Assemble Compact Myelin?Cells · 2020Article
- Flexible Players within the Sheaths: The Intrinsically Disordered Proteins of Myelin in Health and Disease.Cells · 2020Review
- Molecular structure and function of myelin protein P0 in membrane stacking.Scientific reports · 2019Article
- Neuropathy-related mutations alter the membrane binding properties of the human myelin protein P0 cytoplasmic tail.PloS one · 2019Article
- Direct Binding of the Flexible C-Terminal Segment of Periaxin to β4 Integrin Suggests a Molecular Basis for CMT4F.Frontiers in molecular neuroscience · 2019Article
- Pathological transitions in myelin membranes driven by environmental and multiple sclerosis conditions.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line.Scientific reports · 2017Article
- Cholesterol Corrects Altered Conformation of MHC-II Protein in Leishmania donovani Infected Macrophages: Implication in Therapy.PLoS neglected tropical diseases · 2016Article
- Minireview: translocator protein (TSPO) and steroidogenesis: a reappraisal.Molecular endocrinology (Baltimore, Md.) · 2015Review
- Translocator protein/peripheral benzodiazepine receptor is not required for steroid hormone biosynthesis.Endocrinology · 2014Article
- Multiple cholesterol recognition/interaction amino acid consensus (CRAC) motifs in cytosolic C tail of Slo1 subunit determine cholesterol sensitivity of Ca2+- and voltage-gated K+ (BK) channels.The Journal of biological chemistry · 2012Article
- Choline modulation of the aβ p1-40 channel reconstituted into a model lipid membrane.International journal of Alzheimer's disease · 2011Article
- Cholesterol regulates the endoplasmic reticulum exit of the major membrane protein P0 required for peripheral myelin compaction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Peripheral myelin of Xenopus laevis: role of electrostatic and hydrophobic interactions in membrane compaction.Journal of structural biology · 2008Article
- Cytoplasmic domain of zebrafish myelin protein zero: adhesive role depends on beta-conformation.Biophysical journal · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myelin protein zero (P0 or P0 glycoprotein), the major integral membrane protein in peripheral nervous system myelin, plays a key role in myelin membrane compaction and stability. While the structure of P0 extracellular domain was determined by crystallography, the paucity of any structural data on the highly positive-charged P0 cytoplasmic domain (P0-cyt) has greatly limited our understanding of the mechanism of P0 function. Here, using circular dichroism and intrinsic fluorescence spectroscopy, we attempted to elucidate the structure of human P0-cyt (hP0-cyt) in membrane mimetic environments composed of detergents or lipid vesicles. We found that the secondary structure of P0-cyt was polymorphic-at the lipid/protein ratio corresponding to that of mature peripheral myelin ( approximately 50:1), hP0-cyt mainly adopted a beta-conformation, whereas when the proportion of lipid increased, the structure underwent a beta-->alpha transition. By contrast, the secondary structure of the major isoform of myelin basic protein, another myelin protein with a very large positive charge, remained unchanged across a wide range of lipid/protein ratios. We propose that when hP0-cyt is bound at sufficient concentration to lamellar lipid bilayers such as myelin, it folds into a beta-conformation; before this threshold lipid/protein ratio is reached, the domain is alpha-helical. We suggest that the cytoplasmic apposition (major dense line) in compact myelin may be stabilized via the hydrogen-bonding of beta-strands formed as a result of local P0-P0 aggregation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.