ArticleNucleic acids research2006
NHEJ-deficient DT40 cells have increased levels of immunoglobulin gene conversion: evidence for a double strand break intermediate.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Positive Cofactor 4 (PC4) is critical for DNA repair pathway re-routing in DT40 cells.Scientific reports · 2016Article
- C-terminal region of activation-induced cytidine deaminase (AID) is required for efficient class switch recombination and gene conversion.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Checkpoint kinase 2 is required for efficient immunoglobulin diversification.Cell cycle (Georgetown, Tex.) · 2014Article
- Gene Conversion-Like Events in the Diversification of Human Rearranged IGHV3-23*01 Gene Sequences.Frontiers in immunology · 2012Article
- Competition between PARP-1 and Ku70 control the decision between high-fidelity and mutagenic DNA repair.DNA repair · 2011Article
- Incorporation of dUTP does not mediate mutation of A:T base pairs in Ig genes in vivo.Nucleic acids research · 2010Article
- Ubc13 dosage is critical for immunoglobulin gene conversion and gene targeting in vertebrate cells.Nucleic acids research · 2010Article
- Temporal regulation of Ig gene diversification revealed by single-cell imaging.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Genetic evidence for single-strand lesions initiating Nbs1-dependent homologous recombination in diversification of Ig v in chicken B lymphocytes.PLoS genetics · 2009Article
- Brca1 in immunoglobulin gene conversion and somatic hypermutation.DNA repair · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation-induced cytidine deaminase (AID) likely initiates immunoglobulin gene-conversion (GC) by deaminating cytidines within the V-region of chicken B-cells. However, the intervening DNA lesion required to initiate GC remains elusive. GC could be initiated by a single strand break or a double strand break (DSB). To distinguish between these possibilities, we examined GC in the chicken DT40 B cell line deficient in non-homologous end joining (NHEJ). It is known that the NHEJ and homologous recombination DNA repair pathways compete for DSBs. In light of this, if a DSB is the major intermediate, deficiency in NHEJ should result in increased levels of GC. Here we show that DNA-PKcs(-/-/-) and Ku70(-/-) DT40 cells had 5- to 10-fold higher levels of GC relative to wildtype DT40 as measured by surface IgM reversion and sequencing of the V-region. These data suggest that a DSB is the major DNA lesion that initiates GC.
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