ArticleThe Journal of membrane biology1991
Ion channels in the plasma membrane of Amaranthus protoplasts: one cation and one anion channel dominate the conductance.
Article in The Journal of membrane biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 48 citations in OpenAlex.
- A novel Cl- inward-rectifying current in the plasma membrane of the calcifying marine phytoplankton Coccolithus pelagicus.Plant physiology · 2003Article
- A patch-clamp study on the physiology of aluminum toxicity and aluminum tolerance in maize. Identification and characterization of Al(3+)-induced anion channels.Plant physiology · 2001Article
- The delivery of salts to the xylem. Three types of anion conductance in the plasmalemma of the xylem parenchyma of roots of barley.Plant physiology · 2000Article
- Sulfate is both a substrate and an activator of the voltage-dependent anion channel of Arabidopsis hypocotyl cells.Plant physiology · 1999Article
- Kinetic analysis of two simultaneously activated K+ currents in root cell protoplasts of Plantago media L.The Journal of membrane biology · 1995Article
- Anion channels as central mechanisms for signal transduction in guard cells and putative functions in roots for plant-soil interactions.Plant molecular biology · 1995Review
- Green circuits--the potential of plant specific ion channels.Plant molecular biology · 1994Review
- Ion channels in the plasma membrane of protoplasts from the halophytic angiosperm Zostera muelleri.The Journal of membrane biology · 1994Article
- Pump and K+ inward rectifiers in the plasmalemma of wheat root protoplasts.The Journal of membrane biology · 1994Article
- Characterization of ion channels in the plasma membrane of epidermal cells of expanding pea (Pisum sativum arg) leaves.The Journal of membrane biology · 1994Article
- Characterization of ion channels from Acetabularia plasma membrane in planar lipid bilayers.The Journal of membrane biology · 1993Article
- Plasmalemmal, voltage-dependent ionic currents from excitable pulvinar motor cells of Mimosa pudica.The Journal of membrane biology · 1993Article
- Two types of anion channel currents in guard cells with distinct voltage regulation.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Multiple conductances in the large K+ channel from Chara corallina shown by a transient analysis method.Biophysical journal · 1992Article
- The k/na selectivity of a cation channel in the plasma membrane of root cells does not differ in salt-tolerant and salt-sensitive wheat species.Plant physiology · 1991Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report details preliminary findings for ion channels in the plasma membrane of protoplasts derived from the cotyledons of Amaranthus seedlings. The conductance properties of the membrane can be described almost entirely by the behavior of two types of ion channel observed as single channels in attached and detached patches. The first is a cation-selective outward rectifier, and the second a multistate anion-selective channel which, under physiological conditions, acts as an inward rectifier. The cation channel has unit conductance of approx. 30 pS (symmetrical 100 K+) and relative permeability sequence K+ greater than Na+ much greater than Cl- (1:0.16:0.03): whole-cell currents activate in a time-dependent manner, and both activation and deactivation kinetics are voltage dependent. The anion channel opens for hyperpolarized membrane potentials, has a full-level conductance of approx. 200 pS and multiple subconductance states. The number of subconductances does not appear to be fixed. When activated the channel is open for long periods, though shuts if the membrane potential (Vm) is depolarized; at millimolar levels of [Ca2+]cyt this voltage dependency disappears. Inward current attributable to the anion channel is not observed in whole-cell recordings when MgATP (2 mM) is present in the intracellular solution. By contrast the channel is active in most detached patches, whether MgATP is present or not on the cytoplasmic face of the membrane. The anion channel has a significant permeability to cations, the sequence being NO3- greater than Cl- greater than K+ greater than Aspartate (2.04:1:0.18 to 0.09:0.04). The relative permeability for K+ decreased at progressively lower conductance states. In the absence of permeant anions this channel could be mistaken for a cation inward rectifier. The anion and cation channels could serve to clamp Vm at a preferred value in the face of events which would otherwise perturb Vm.
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