ArticleThe EMBO journal2006
DNA segregation by the bacterial actin AlfA during Bacillus subtilis growth and development.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Polymer dynamics of Alp7A reveals how two critical concentrations govern assembly of dynamically unstable actin-like proteins.Molecular biology of the cell · 2024Article
- Archaeal actins and the origin of a multi-functional cytoskeleton.Journal of bacteriology · 2024Review
- Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions.Chemical reviews · 2024Review
- The alternative sigma factor SigN ofJournal of bacteriology · 2023Article
- Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.Frontiers in microbiology · 2022Review
- Article
- Application of Bacillus subtilis as a live vaccine vector: A review.The Journal of veterinary medical science · 2020Review
- The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis.Journal of bacteriology · 2020Review
- Article
- Prokaryotic cytoskeletons: protein filaments organizing small cells.Nature reviews. Microbiology · 2018Review
- Cryo-EM structure of the bacterial actin AlfA reveals unique assembly and ATP-binding interactions and the absence of a conserved subdomain.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The complex simplicity of the bacterial cytoskeleton.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cryo-EM reconstruction of AlfA fromProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Review
- Polymerization Dynamics of the Prophage-Encoded Actin-Like Protein AlpC Is Influenced by the DNA-Binding Adapter AlpA.Frontiers in microbiology · 2017Article
- Determinants of Bacterial Morphology: From Fundamentals to Possibilities for Antimicrobial Targeting.Frontiers in microbiology · 2017Review
- ZpdN, a Plasmid-Encoded Sigma Factor Homolog, Induces pBS32-Dependent Cell Death in Bacillus subtilis.Journal of bacteriology · 2016Article
- Segregation of prokaryotic magnetosomes organelles is driven by treadmilling of a dynamic actin-like MamK filament.BMC biology · 2016Article
- Localization of Low Copy Number Plasmid pRC4 in Replicating Rod and Non-Replicating Cocci Cells of Rhodococcus erythropolis PR4.PloS one · 2016Article
- Article
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Authors and funding
8 authors.
Funding
Abstract
We here identify a protein (AlfA; actin like filament) that defines a new family of actins that are only distantly related to MreB and ParM. AlfA is required for segregation of Bacillus subtilis plasmid pBET131 (a mini pLS32-derivative) during growth and sporulation. A 3-kb DNA fragment encoding alfA and a downstream gene (alfB) is necessary and sufficient for plasmid stability. AlfA-GFP assembles dynamic cytoskeletal filaments that rapidly turn over (t(1/2)< approximately 45 s) in fluorescence recovery after photobleaching experiments. A point mutation (alfA D168A) that completely inhibits AlfA subunit exchange in vivo is strongly defective for plasmid segregation, demonstrating that dynamic polymerization of AlfA is necessary for function. During sporulation, plasmid segregation occurs before septation and independently of the DNA translocase SpoIIIE and the chromosomal Par proteins Soj and Spo0J. The absence of the RacA chromosome anchoring protein reduces the efficiency of plasmid segregation (by about two-fold), suggesting that it might contribute to anchoring the plasmid at the pole during sporulation. Our results suggest that the dynamic polymerization of AlfA mediates plasmid separation during both growth and sporulation.
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