ArticleBlood2007
The effect of deoxygenation on whole-cell conductance of red blood cells from healthy individuals and patients with sickle cell disease.
Article in Blood, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Pooled it
- Simulated Microgravity Induces Rapid Dielectric Shifts in Erythrocytes of Pancreatic Cancer Patients.ACS nanoscience Au · 2026Article
- Breaking Free From MCHC Interferences? French-Speaking Cellular Haematology Group (GFHC) Review of Causes, Rising Trends and Practical Solutions.International journal of laboratory hematology · 2025Review
- Activating pyruvate kinase improves red blood cell integrity by reducing band 3 tyrosine phosphorylation.Blood advances · 2024Article
- Gene expression changes in sickle cell reticulocytes and their clinical associations.Scientific reports · 2023Article
- Rapid degradation of protein tyrosine phosphatase 1B in sickle cells: Possible contribution to sickle cell membrane weakening.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Article
- Ten-Second Electrophysiology: Evaluation of the 3DEP Platform for high-speed, high-accuracy cell analysis.Scientific reports · 2019Article
- Disorders of erythrocyte hydration.Blood · 2017Review
- Calcium in red blood cells-a perilous balance.International journal of molecular sciences · 2013Review
- Harnessing red cell membrane pathophysiology towards point-of-care diagnosis for sickle cell disease.The Journal of physiology · 2013Article
- Mutations in the mechanotransduction protein PIEZO1 are associated with hereditary xerocytosis.Blood · 2012Article
- The conductance of red blood cells from sickle cell patients: ion selectivity and inhibitors.The Journal of physiology · 2012Article
- The Properties of Red Blood Cells from Patients Heterozygous for HbS and HbC (HbSC Genotype).Anemia · 2011Article
- Inhibition of cation channels in human erythrocytes by spermine.The Journal of membrane biology · 2010Article
- Article
- Reduced DIDS-sensitive chloride conductance in Ae1-/- mouse erythrocytes.Blood cells, molecules & diseasesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Red blood cells from patients with sickle cell disease (SCD) exhibit increased electrogenic cation permeability, particularly following deoxygenation and hemoglobin (Hb) polymerisation. This cation permeability, termed P(sickle), contributes to cellular dehydration and sickling, and its inhibition remains a major goal for SCD treatment. Nevertheless, its characteristics remain poorly defined, its molecular identity is unknown, and effective inhibitors have not been established. Here, patch-clamp methodology was used to record whole-cell currents in single red blood cells from healthy individuals and patients with SCD. Oxygenated normal red blood cells had a low membrane conductance, unaffected by deoxygenation. Oxygenated HbS cells had significantly increased conductance and, on deoxygenation, showed a further rise in membrane conductance. The deoxygenation-induced pathway was variable in magnitude. It had equal permeability to Na(+) and K(+), but was less permeable to NMDG(+) and Cl(-). Conductance to Ca(2+) was also of a similar magnitude to that of monovalent cations. It was inhibited by DIDS (100 microM), Zn(2+) (100 microM), and by Gd(3+) (IC(50) of approximately 2 microM). It therefore shares some properties with P(sickle). These findings represent the first electrical recordings of single HbS cells and will facilitate progress in understanding altered red blood cell cation transport characteristics of SCD.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.