ArticleRNA (New York, N.Y.)2007
The position of yeast snoRNA-coding regions within host introns is essential for their biosynthesis and for efficient splicing of the host pre-mRNA.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Comprehensive analysis of Saccharomyces cerevisiae intron structures in vivo.Nature structural & molecular biology · 2025Article
- Motif conservation, stability, and host gene expression are the main drivers of snoRNA expression across vertebrates.Genome research · 2023Article
- Article
- Emerging Data on the Diversity of Molecular Mechanisms Involving C/D snoRNAs.Non-coding RNA · 2021Review
- The cellular landscape of mid-size noncoding RNA.Wiley interdisciplinary reviews. RNA · 2019Review
- 5'-End sequencing in Saccharomyces cerevisiae offers new insights into 5'-ends of tRNAFEBS letters · 2019Article
- Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis.RNA (New York, N.Y.) · 2019Article
- Paralog-Specific Functions ofG3 (Bethesda, Md.) · 2017Article
- Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts.PloS one · 2012Article
- Untangling the phenotypic heterogeneity of Diamond Blackfan anemia.Seminars in hematology · 2011Review
- SnoRNAs from the filamentous fungus Neurospora crassa: structural, functional and evolutionary insights.BMC genomics · 2009Article
- Incorporating functional inter-relationships into protein function prediction algorithms.BMC bioinformatics · 2009Article
- Analysis of small nucleolar RNAs reveals unique genetic features in malaria parasites.BMC genomics · 2009Article
- An evolutionary perspective of animal microRNAs and their targets.Journal of biomedicine & biotechnology · 2009Review
- On the evolution and expression of Chlamydomonas reinhardtii nucleus-encoded transfer RNA genes.Genetics · 2008Article
- Article
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4 authors at 2 institutions in 1 country.
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Abstract
Genomic location of sequences encoding small nucleolar RNAs (snoRNAs) is peculiar in all eukaryotes from yeast to mammals: most of them are encoded within the introns of host genes. In Saccharomyces cerevisiae, seven snoRNAs show this location. In this work we demonstrate that the position of snoRNA-coding regions with respect to splicing consensus sequences is critical: yeast strains expressing mutant constructs containing shorter or longer spacers (the regions between snoRNA ends and intron splice sites) show a drop in accumulation of U24 and U18 snoRNAs. Further mutational analysis demonstrates that altering the distance between the 3' end of the snoRNA and the branch point is the most important constraint for snoRNA biosynthesis, and that stable external stems, which are sometimes present in introns containing snoRNAs, can overcome the positional effect. Surprisingly enough, splicing of the host introns is clearly affected in most of these constructs indicating that, at least in S. cerevisiae, an incorrect location of snoRNA-coding sequences within the host intron is detrimental to the splicing process. This is different with respect to what was demonstrated in mammals, where the activity of the splicing machinery seems to be dominant with respect to the assembly of snoRNPs, and it is not affected by the location of snoRNA sequences. We also show that intronic box C/D snoRNA recognition and assembly of snoRNPs occur during transcription when splicing sequences are recognized.
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