ArticleJournal of virology2007
A novel repeat-associated small interfering RNA-mediated silencing pathway downregulates complementary sense gypsy transcripts in somatic cells of the Drosophila ovary.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 139 citations in OpenAlex.
- Retrovirus insertions in host transcripts trigger de novo piRNA immunity.The EMBO journal · 2026Article
- Co-evolving infectivity and expression patterns drive the diversification of endogenous retroviruses.The EMBO journal · 2026Article
- Target-Directed miRNA Degradation: Mechanisms and Significance.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
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- Terminal modification, sequence, length, and PIWI-protein identity determine piRNA stability.Molecular cell · 2021Article
- Satellite-Like W-Elements: Repetitive, Transcribed, and Putative Mobile Genetic Factors with Potential Roles for Biology and Evolution of Schistosoma mansoni.Genome biology and evolution · 2021Article
- RNAs on the Go: Extracellular Transfer in Insects with Promising Prospects for Pest Management.Plants (Basel, Switzerland) · 2021Review
- piRNAs as Modulators of Disease Pathogenesis.International journal of molecular sciences · 2021Review
- Somatic piRNAs and Transposons are Differentially Expressed Coincident with Skeletal Muscle Atrophy and Programmed Cell Death.Frontiers in genetics · 2021Article
- Special vulnerability of somatic niche cells to transposable element activation in Drosophila larval ovaries.Scientific reports · 2020Article
- Functional lability of RNA-dependent RNA polymerases in animals.PLoS genetics · 2019Article
- PIWI-interacting RNAs: small RNAs with big functions.Nature reviews. Genetics · 2019Review
- Evolution and biological significance of flaviviral elements in the genome of the arboviral vectorEmerging microbes & infections · 2019Article
- Endogenous non-retroviral elements in genomes of Aedes mosquitoes and vector competence.Emerging microbes & infections · 2019Review
- The somatic piRNA pathway controls germline transposition over generations.Nucleic acids research · 2018Article
- The genome of the Hi5 germ cell line fromeLife · 2018Article
- Target RNAs Strike Back on MicroRNAs.Frontiers in genetics · 2018Review
- piRNA pathway gene expression in the malaria vector mosquito Anopheles stephensi.Insect molecular biology · 2014Article
- Article
20 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replication of the gypsy endogenous retrovirus involves contamination of the female germ line by adjacent somatic tissues. This is prevented by flam, an as-yet-uncloned heterochromatic pericentromeric locus, at the level of transcript accumulation in these somatic ovarian tissues. We tested the effect of a presumptive RNA silencing mechanism on the accumulation of RNAs produced by constructs containing various gypsy sequences and report that the efficiency of silencing is indeed correlated with the amount of complementary RNAs, 25 to 30 nucleotides in length, in the ovary. For instance, while these RNAs were found to display a three- to fivefold excess of the antisense strands, only the transcripts that contain the complementary sense gypsy sequences could be repressed, indicating that they are targeted at the RNA, not DNA, level. Their size and asymmetry in strand polarity are typical of the novel repeat-associated small interfering RNA (rasiRNA)-mediated pathway, recently suspected to prevent the deleterious expression of selfish DNA specifically in the germ line. Unlike microRNAs (but like rasiRNAs and, surprisingly, siRNAs as well), gypsy rasiRNAs are modified at the 3' end. The rasiRNA-associated protein Piwi (but not Aub) is required for gypsy silencing, whereas Dicer-2 (which makes siRNAs) is not. In contrast, piwi, aub, and flam do not appear to affect somatic siRNA-mediated silencing. The amount of gypsy rasiRNAs is genetically determined by the flam locus in a provirus copy number-independent manner and is triggered in the somatic tissues by some pericentromeric provirus(es), which are thereby able to protect the germ line from retroviral invasion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.