ArticleJournal of virology2007
Genome sequence, full-length infectious cDNA clone, and mapping of viral double-stranded RNA accumulation determinant of hypovirus CHV1-EP721.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- Hypovirus-Induced Phosphorylation of CpIre1 Modulates Unfolded Protein Response and Virulence in Cryphonectria parasitica.Molecular plant pathology · 2026Article
- Changes in intra-host mycovirus population diversity after vertical and horizontal transmission.Virus evolution · 2025Article
- Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Deoxynivalenol Biosynthesis inToxins · 2022Article
- Long-read sequencing reveals the evolutionary drivers of intra-host diversity across natural RNA mycovirus infections.Virus evolution · 2021Article
- A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions.Science advances · 2020Article
- Virome Identification and Characterization ofFrontiers in microbiology · 2020Article
- Mycoviruses inFrontiers in cellular and infection microbiology · 2019Review
- CpATG8, a Homolog of Yeast Autophagy Protein ATG8, Is Required for Pathogenesis and Hypovirus Accumulation in the Chest Blight Fungus.Frontiers in cellular and infection microbiology · 2019Article
- Comparative Methylome Analysis Reveals Perturbation of Host Epigenome in Chestnut Blight Fungus by a Hypovirus.Frontiers in microbiology · 2018Article
- Cryphonectria parasitica, the causal agent of chestnut blight: invasion history, population biology and disease control.Molecular plant pathology · 2018Review
- Article
- Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus.Scientific reports · 2016Article
- Transfection of Sclerotinia sclerotiorum with in vitro transcripts of a naturally occurring interspecific recombinant of Sclerotinia sclerotiorum hypovirus 2 significantly reduces virulence of the fungus.Journal of virology · 2015Article
- Thyreophagus corticalis as a vector of hypovirulence in Cryphonectria parasitica in chestnut stands.Experimental & applied acarology · 2014Article
- Variations in hypovirus interactions with the fungal-host RNA-silencing antiviral-defense response.Journal of virology · 2012Article
- Characterization of a novel bipartite double-stranded RNA mycovirus conferring hypovirulence in the phytopathogenic fungus Botrytis porri.Journal of virology · 2012Article
- Functional categorization of unique expressed sequence tags obtained from the yeast-like growth phase of the elm pathogen Ophiostoma novo-ulmi.BMC genomics · 2011Article
- CYP1, a hypovirus-regulated cyclophilin, is required for virulence in the chestnut blight fungus.Molecular plant pathology · 2011Article
- Mycoviruses, RNA silencing, and viral RNA recombination.Advances in virus research · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Cryphonectria parasitica strain EP721 is infected with a strain of hypovirus CHV1, CHV1-EP721, and exhibits typical hypovirulence-associated traits such as reduced pigmentation and reduced asexual sporulation. However, the accumulation of the viral double-stranded RNA (dsRNA) in this hypovirus-infected C. parasitica strain is atypically low. We now report the complete nucleotide sequence and construction of a full-length infectious cDNA clone for hypovirus CHV1-EP721. The genome sequence of CHV1-EP721 was determined to be 12,724 bp in length and to share extensive homology with two other hypovirus strains, CHV1-Euro7 and CHV1-EP713, with an average of 99% and 90% identities at the nucleotide level and 99% and 92% identities at the amino acid level, respectively. CHV1-EP721 was successfully introduced into virus-free fungal host strain EP721(-v) by transfection with transcripts derived from a full-length viral cDNA. The transfected strain had a phenotype indistinguishable from that of EP721, and the accumulation of CHV1-EP721 dsRNA in the transfectant was lower than those transfected by CHV1-Euro7 and CHV1-EP713 transcripts. Through the construction of chimeric viruses by domain swapping using infectious cDNA clones of CHV1-EP721, CHV1-EP713, and CHV1-Euro7 hypoviruses, the determinant for the low level of viral dsRNA accumulation in CHV1-EP721 was mapped to the second of two CHV1-EP721 open reading frames (ORFs), ORF B. Further refined swapping of domains within ORF B identified a 2.5-kb coding region between p48 and the polymerase domain of CHV1-EP721 as being responsible for the low viral dsRNA accumulation. Evidence is also provided that low rates of hypovirus transmission through conidial spores correlates with low viral dsRNA accumulation.
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