ArticleJournal of molecular biology2007
Molecular mechanism of the regulation of Bacillus subtilis gltAB expression by GltC.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 86 citations in OpenAlex.
- Article
- Galactose-1-phosphate uridylyltransferase GalT promotes biofilm formation and enhances UV-B resistance of Bacillus thuringiensis.World journal of microbiology & biotechnology · 2024Article
- Fundamentals and Exceptions of the LysR-type Transcriptional Regulators.ACS synthetic biology · 2024Review
- MoBioS: Modular Platform Technology for High-Throughput Construction and Characterization of Tunable Transcriptional Biological Sensors.Biosensors · 2023Article
- Genome-wide mapping of GlnR-binding sites reveals the global regulatory role of GlnR in controlling the metabolism of nitrogen and carbon in Paenibacillus polymyxa WLY78.BMC genomics · 2023Article
- Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity.Medical sciences (Basel, Switzerland) · 2022Review
- A counter-enzyme complex regulates glutamate metabolism in Bacillus subtilis.Nature chemical biology · 2022Article
- Functional and structural analysis of catabolite control protein C that responds to citrate.Scientific reports · 2021Article
- The LysR-Type Transcriptional Regulator BsrA (PA2121) Controls Vital Metabolic Pathways inmSystems · 2021Article
- A LysR Family Transcriptional Regulator Modulates Burkholderia cenocepacia Biofilm Formation and Protease Production.Applied and environmental microbiology · 2021Article
- McbG, a LysR Family Transcriptional Regulator, Activates theApplied and environmental microbiology · 2021Article
- A novel LysR-type regulator negatively affects biosynthesis of the immunosuppressant brasilicardin.Engineering in life sciences · 2021Article
- Role of GlnR in Controlling Expression of Nitrogen Metabolism Genes inJournal of bacteriology · 2020Article
- Article
- Role of Glutamate Synthase in Biofilm Formation by Bacillus subtilis.Journal of bacteriology · 2020Article
- Article
- Variants of theFrontiers in microbiology · 2019Article
- Biosensors-Based In Vivo Quantification of 2-Oxoglutarate in Cyanobacteria and Proteobacteria.Life (Basel, Switzerland) · 2018Article
- Positive and negative regulation of transferred nif genes mediated by indigenous GlnR in Gram-positive Paenibacillus polymyxa.PLoS genetics · 2018Article
- Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis.Molecular microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In Bacillus subilis, glutamate synthase, a major enzyme of nitrogen metabolism, is encoded by the gltAB operon. Significant expression of this operon requires the activity of GltC, a LysR-family protein, encoded by the divergently transcribed gene. We purified a soluble, active form of GltC and found that it requires alpha-ketoglutarate, a substrate of glutamate synthase, to fully activate gltA transcription in vitro, and that its activity is inhibited by glutamate, the product of glutamate synthase. GltC regulated gltAB transcription through binding to three dyad-symmetry elements, Box I, Box II and Box III, located in the intergenic region of gltC and gltA. Free GltC bound almost exclusively to Box I and activated gltAB transcription only marginally. Glutamate-bound GltC bound to Box I and Box III, and repressed gltAB transcription. In the presence of alpha-ketoglutarate, GltC bound to Box I and Box II, stabilized binding of RNA polymerase to the gltA promoter, and activated gltAB transcription. The binding of GltC to Box II, which partially overlaps the -35 region of the gltA promoter, seems to be essential for activation of the gltAB operon. Due to the high concentration of glutamate in B. subtilis cells grown under most conditions, alterations of the concentration of alpha-ketoglutarate seem to be crucial for modulation of GltC activity and gltAB expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.