ArticleHistochemistry1991
Heterogeneity in the immunolocalization of cytokeratin specific monoclonal antibodies in the rat eye: evaluation of unusual epithelial tissue entities.
Article in Histochemistry, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies.Translational vision science & technology · 2022Article
- Molecular markers for corneal epithelial cells in larval vs. adult Xenopus frogs.Experimental eye research · 2019Article
- Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators.Scientific reports · 2018Article
- Artificial Polymeric Scaffolds as Extracellular Matrix Substitutes for Autologous Conjunctival Goblet Cell Expansion.Investigative ophthalmology & visual science · 2016Article
- Tcf7l2 localization of putative stem/progenitor cells in mouse conjunctiva.American journal of physiology. Cell physiology · 2016Article
- Interaction of IFN-γ with cholinergic agonists to modulate rat and human goblet cell function.Mucosal immunology · 2016Article
- Potential candidate cells for constructing tissue-engineered lacrimal duct epithelium: a histological and cytological study in rabbits.Journal of Zhejiang University. Science. B · 2015Article
- Biopsy harvesting site and distance from the explant affect conjunctival epithelial phenotype ex vivo.Experimental eye research · 2012Article
- Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.Journal of cellular physiology · 2010Article
- Abnormal epithelial homeostasis in the cornea of mice with a destrin deletion.Molecular vision · 2008Article
- Development of immortalized rat conjunctival epithelial cell lines: an in vitro model to examine transepithelial antigen delivery.Experimental eye research · 2007Article
- Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.The British journal of ophthalmology · 2003Article
- Characterization of epithelial primary cultures from human conjunctiva.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 1997Article
- Heterogeneity in the immunolocalization of cytokeratin-specific monoclonal antibodies in the rat lung: evaluation of three different alveolar epithelial cell types.Histochemistry · 1993Article
- Changes in cytokeratin expression accompany squamous metaplasia of the human respiratory epithelium.Virchows Archiv. A, Pathological anatomy and histopathology · 1992Article
- Regional differences in the distribution of cytoskeletal filaments in the human and bovine ciliary epithelium.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 1992Article
Corrections and comments
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Authors and funding
1 author.
Funding
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Abstract
The immunocytochemical localization of cytokeratin and vimentin in rat eye tissues was investigated using a panel of 39 monoclonal antibodies specific for single or multiple of cytokeratin polypeptides and one polyclonal anti CK20 antiserum. The retinal and the ciliary body pigment epithelial only expressed cytokeratins 8 and 18, whereas the fetal retinal pigment epithelium and focally the adult epithelium, in the transition zone of retina and ciliary body, exhibited a reactivity for cytokeratin 19. In contrast, the non-pigmented ciliary epithelium was positive for vimentin only. In the rat conjunctiva distributed goblet cell clusters were selectively stained with cytokeratin 7, 8, 18 and 19 specific monoclonal antibodies. Among them a group of cytokeratin 8 and 18 specific monoclonal antibodies which stained the goblet cells as well as cytokeratin 8 and 18 positive internal controls did not react with either the cytokeratin 8 and 18 positive neuroectodermal cells of the rat eye nor the rat choroid plexus epithelium. This indicates differences in the phenotype e.g. conformational epitope changes, of neuroectodermal derived and other cytokeratins. The corneal and conjunctival epithelium showed a more complex distribution of squamous epithelium type cytokeratins. The limbal region as a transient zone connecting both epithelia exhibited a changing cytokeratin pattern. In general, the study emphasized the necessity to work with an enlarged antibody panel to avoid misleading results in the immunolocalization of cytokeratins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.