ArticleMolecular and cellular biology2007
Caspase-3 regulates catalytic activity and scaffolding functions of the protein tyrosine phosphatase PEST, a novel modulator of the apoptotic response.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Mapping enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons.Nature methods · 2024Article
- Fas ligand intracellular signaling: does PSTPIP mediate T cell death?Apoptosis : an international journal on programmed cell death · 2024Article
- PSTPIP2 inhibits cisplatin-induced acute kidney injury by suppressing apoptosis of renal tubular epithelial cells.Cell death & disease · 2020Article
- Clinical implications of a novel prognostic factor AIFM3 in breast cancer patients.BMC cancer · 2019Article
- Mammalian Sterile20-like Kinases: Signalings and Roles in Central Nervous System.Aging and disease · 2018Review
- Role of CSPG receptor LAR phosphatase in restricting axon regeneration after CNS injury.Neurobiology of disease · 2015Article
- Selective expression of CSPG receptors PTPσ and LAR in poorly regenerating reticulospinal neurons of lamprey.The Journal of comparative neurology · 2014Article
- Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility.The Journal of biological chemistry · 2013Article
- Regulation of tumor cell migration by protein tyrosine phosphatase (PTP)-proline-, glutamate-, serine-,and threonine-rich sequence (PEST).Chinese journal of cancer · 2013Review
- SHP-2 and PTP-pest induction during Rb-E2F associated apoptosis.Cellular & molecular biology letters · 2012Article
- Tyrosine dephosphorylation is required for Bak activation in apoptosis.The EMBO journal · 2010Article
- Clinical, Molecular, and Genetic Characteristics of PAPA Syndrome: A Review.Current genomics · 2010Article
- F-actin-binding domain of c-Abl regulates localized phosphorylation of C3G: role of C3G in c-Abl-mediated cell death.Oncogene · 2010Article
- PTP-PEST controls motility, adherens junction assembly, and Rho GTPase activity in colon cancer cells.American journal of physiology. Cell physiology · 2010Article
- Protein tyrosine phosphatases expression during development of mouse superior colliculus.Experimental brain research · 2009Article
- The Leishmania surface protease GP63 cleaves multiple intracellular proteins and actively participates in p38 mitogen-activated protein kinase inactivation.The Journal of biological chemistry · 2009Article
- Allele-specific inhibition of divergent protein tyrosine phosphatases with a single small molecule.Bioorganic & medicinal chemistry · 2008Article
- TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.Molecular immunology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protein tyrosine phosphatase PEST (PTP-PEST) is involved in the regulation of the actin cytoskeleton. Despite the emerging functions attributed to both PTPs and the actin cytoskeleton in apoptosis, the involvement of PTP-PEST in apoptotic cell death remains to be established. Using several cell-based assays, we showed that PTP-PEST participates in the regulation of apoptosis. As apoptosis progressed, a pool of PTP-PEST localized to the edge of retracting lamellipodia. Expression of PTP-PEST also sensitized cells to receptor-mediated apoptosis. Concertedly, specific degradation of PTP-PEST was observed during apoptosis. Pharmacological inhibitors, immunodepletion experiments, and in vitro cleavage assays identified caspase-3 as the primary regulator of PTP-PEST processing during apoptosis. Caspase-3 specifically cleaved PTP-PEST at the (549)DSPD motif and generated fragments, some of which displayed increased catalytic activity. Moreover, caspase-3 regulated PTP-PEST interactions with paxillin, leupaxin, Shc, and PSTPIP. PTP-PEST acted as a scaffolding molecule connecting PSTPIP to additional partners: paxillin, Shc, Csk, and activation of caspase-3 correlated with the modulation of the PTP-PEST adaptor function. In addition, cleavage of PTP-PEST facilitated cellular detachment during apoptosis. Together, our data demonstrate that PTP-PEST actively contributes to the cellular apoptotic response and reveal the importance of caspases as regulators of PTPs in apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.