ArticleNucleic acids research2006
Self-splicing of a group IIC intron: 5' exon recognition and alternative 5' splicing events implicate the stem-loop motif of a transcriptional terminator.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Recurrent Modular Assembly Drives Independent Origins of Group II Introns Encoding LAGLIDADG Homing Endonucleases.Genome biology and evolution · 2026Article
- Cysteine Mutagenesis of a Group II Intron-Encoded Protein Supports Splicing, Mobility, and Site-Specific Labeling.Biochemistry · 2025Article
- Structures of a natural circularly permuted group II intron reveal mechanisms of branching and backsplicing.Nature structural & molecular biology · 2025Article
- Group IIC self-splicing intron-derived novel circular RNA vaccine elicits superior immune response against RSV.Frontiers in immunology · 2025Article
- Targeting the conserved active site of splicing machines with specific and selective small molecule modulators.Nature communications · 2024Article
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- Pairwise Engineering of Tandemly Aligned Self-Splicing Group I Introns for Analysis and Control of Their Alternative Splicing.Biomolecules · 2023Article
- Structures of a mobile intron retroelement poised to attack its structured DNA target.Science (New York, N.Y.) · 2022Article
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- Group II intron as cold sensor for self-preservation and bacterial conjugation.Nucleic acids research · 2020Article
- Transitions between the steps of forward and reverse splicing of group IIC introns.RNA (New York, N.Y.) · 2020Article
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- A Highly Proliferative Group IIC Intron from Geobacillus stearothermophilus Reveals New Features of Group II Intron Mobility and Splicing.Journal of molecular biology · 2018Article
- Structure determination of group II introns.Methods (San Diego, Calif.) · 2017Review
- Activating the branch-forming splicing pathway by reengineering the ribozyme component of a natural group II intron.RNA (New York, N.Y.) · 2016Article
- The Ll.LtrB intron from Lactococcus lactis excises as circles in vivo: insights into the group II intron circularization pathway.RNA (New York, N.Y.) · 2015Article
- Mobile Bacterial Group II Introns at the Crux of Eukaryotic Evolution.Microbiology spectrum · 2015Review
- Group II intron lariat: Structural insights into the spliceosome.RNA biology · 2015Review
- In vitro characterization of the splicing efficiency and fidelity of the RmInt1 group II intron as a means of controlling the dispersion of its host mobile element.RNA (New York, N.Y.) · 2014Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Bacterial IIC introns are a newly recognized subclass of group II introns whose ribozyme properties have not been characterized in detail. IIC introns are typically located downstream of transcriptional terminator motifs (inverted repeat followed by T's) or other inverted repeats in bacterial genomes. Here we have characterized the self-splicing activity of a IIC intron, B.h.I1, from Bacillus halodurans. B.h.I1 self-splices in vitro through hydrolysis to produce linear intron, but interestingly, additional unexpected products were formed that were highly dependent on ionic conditions. These products were determined to represent alternative splicing events at the 5' junction and cleavages throughout the RNA transcript. The alternative splicing and cleavage events occurred at cryptic splice sites containing stem-loop and IBS1 motifs, suggesting that the 5' exon is recognized by both elements. These results provide the first example of a group II intron that uses 5' splice sites nonadjacent to the ribozyme structure. Furthermore, the data suggest that IIC introns differ from IIA and IIB introns with respect to 5' exon definition, and that the terminator stem-loop substitutes in part for the missing IBS2-EBS2 (intron and exon binding sites 2) interaction.
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