ArticleMolecular and biochemical parasitology2007
Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: evidence for a role in hookworm reproductive physiology.
Article in Molecular and biochemical parasitology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- High-Fat Diets Disturb Rat Epididymal Sperm Maturation.International journal of molecular sciences · 2025Article
- Protein profile of extracellular vesicles derived from adult Parascaris spp.Parasites & vectors · 2024Article
- Identification of Male Sex-Related Genes Regulated byInternational journal of molecular sciences · 2023Article
- Excretory/Secretory Proteome of Females and Males of the HookwormPathogens (Basel, Switzerland) · 2023Article
- Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study ofBiology · 2021Article
- Twenty-five-year research progress in hookworm excretory/secretory products.Parasites & vectors · 2020Review
- Let's talk about sexes: sex-related N-glycosylation in ecologically important invertebrates.Glycoconjugate journal · 2020Review
- Sex and tissue specific gene expression patterns identified following de novo transcriptomic analysis of the Norway lobster, Nephrops norvegicus.BMC genomics · 2017Article
- Molecular cloning and characterization of a nematode polyprotein antigen/allergen from the human and animal hookworm Ancylostoma ceylanicum.Molecular and biochemical parasitology · 2014Article
- Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum.Molecular biology reports · 2014Article
- A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system.BMB reports · 2013Article
- LEC-2, a highly variable lectin in the lichenSymbiosis (Philadelphia, Pa.) · 2012Article
- A novel C-type lectin identified by EST analysis in tissue migratory larvae of Ascaris suum.Parasitology research · 2012Article
- C-type lectins from the nematode parasites Heligmosomoides polygyrus and Nippostrongylus brasiliensis.Parasitology international · 2009Article
- Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum.International journal for parasitology · 2009Article
- Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity.Molecular and biochemical parasitology · 2009Review
- Structural and functional characterization of a secreted hookworm Macrophage Migration Inhibitory Factor (MIF) that interacts with the human MIF receptor CD74.The Journal of biological chemistry · 2007Article
- Phylogenetic Analysis of C Type Lectin fromIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Lectins comprise a family of related proteins that mediate essential cell functions through binding to carbohydrates. Within this protein family, C-type lectins are defined by the requirement of calcium for optimal biologic activity. Using reverse transcription PCR, a cDNA corresponding to a putative C-type lectin has been amplified from the hookworm parasite Ancylostoma ceylanicum. The 550 nucleotide open reading frame of the A. ceylanicum C-type Lectin-1 (AceCTL-1) cDNA corresponds to a 167 amino acid mature protein (18,706 Da) preceded by a 17 amino acid secretory signal sequence. The recombinant protein (rAceCTL-1) was expressed in Drosophila S2 cells and purified using a combination of affinity chromatography and reverse phase HPLC. Using in vitro carbohydrate binding studies, it was determined that rAceCTL-1 binds N-acetyl-d-glucosamine, a common component of eukaryotic egg cell membranes. Using a polyclonal IgG raised against the recombinant protein, the native AceCTL-1 was identified in sperm and soluble protein extracts of adult male A. ceylanicum by immunoblot. Probing of adult hookworm sections with the polyclonal IgG demonstrated localization to the testes in males, as well as the spermatheca and developing embryos in females, consistent with its role as a sperm protein. Together, these data strongly suggest that AceCTL-1 is a male gender-specific C-type lectin with a function in hookworm reproductive physiology.
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