ArticleJournal of virology1991
Spreading of DNA methylation across integrated foreign (adenovirus type 12) genomes in mammalian cells.
Article in Journal of virology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 65 citations in OpenAlex.
- DNA methylation at hepatitis B virus integrants and flanking host mitochondrially encoded cytochrome C oxidase III.Oncology letters · 2022Article
- Silencing of Unintegrated Retroviral DNAs.Viruses · 2021Review
- HPV integration hijacks and multimerizes a cellular enhancer to generate a viral-cellular super-enhancer that drives high viral oncogene expression.PLoS genetics · 2018Article
- Identification of human papillomavirus (HPV) 16 DNA integration and the ensuing patterns of methylation in HPV-associated head and neck squamous cell carcinoma cell lines.International journal of cancer · 2017Article
- DNA methylation at hepatitis B viral integrants is associated with methylation at flanking human genomic sequences.Genome research · 2015Article
- DNA cytosine methylation and hydroxymethylation at the borders.Epigenomics · 2014Article
- The inherent processivity of the human de novo methyltransferase 3A (DNMT3A) is enhanced by DNMT3L.The Journal of biological chemistry · 2010Article
- DNA hypomethylation in cancer cells.Epigenomics · 2009Review
- Presence and role of cytosine methylation in DNA viruses of animals.Nucleic acids research · 2008Review
- Insertion of foreign DNA into an established mammalian genome can alter the methylation of cellular DNA sequences.Journal of virology · 1999Article
- Gene silencing by DNA methylation and dual inheritance in Chinese hamster ovary cells.Genetics · 1998Article
- Mapping promoter regions that are hypersensitive to methylation-mediated inhibition of transcription: application of the methylation cassette assay to the Epstein-Barr virus major latency promoter.Journal of virology · 1997Article
- Spreading of methylation along DNA.The Biochemical journal · 1996Article
- The structure of adenovirus type 12 DNA integration sites in the hamster cell genome.Journal of virology · 1996Article
- The initiation of de novo methylation of foreign DNA integrated into a mammalian genome is not exclusively targeted by nucleotide sequence.Journal of virology · 1995Article
- Ingested foreign (phage M13) DNA survives transiently in the gastrointestinal tract and enters the bloodstream of mice.Molecular & general genetics : MGG · 1994Article
- Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis.Journal of virology · 1994Article
- Selective sites of adenovirus (foreign) DNA integration into the hamster genome: changes in integration patterns.Journal of virology · 1994Article
- Inactive chromatin spreads from a focus of methylation.Molecular and cellular biology · 1993Article
- Patterns of frog virus 3 DNA methylation and DNA methyltransferase activity in nuclei of infected cells.Journal of virology · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The establishment of de novo-generated patterns of DNA methylation is characterized by the gradual spreading of DNA methylation (I. Kuhlmann and W. Doerfler, J. Virol. 47:631-636, 1983; M. Toth, U. Lichtenberg, and W. Doerfler, Proc. Natl. Acad. Sci. USA 86:3728-3732, 1989; M. Toth, U. Müller, and W. Doerfler J. Mol. Biol. 214:673-683, 1990). We have used integrated adenovirus type 12 (Ad12) genomes in hamster tumor cells as a model system to study the mechanism of de novo DNA methylation. Ad12 induces tumors in neonate hamsters, and the viral DNA is integrated into the hamster genome, usually nearly intact and in an orientation that is colinear with that of the virion genome. The integrated Ad12 DNA in the tumor cells is weakly methylated at the 5'-CCGG-3' sequences. These sequences appear to be a reliable indicator for the state of methylation in mammalian DNA. Upon explantation of the tumor cells into culture medium, DNA methylation at 5'-CCGG-3' sequences gradually spreads across the integrated viral genomes with increasing passage numbers of cells in culture. Methylation is reproducibly initiated in the region between 30 and 75 map units on the integrated viral genome and progresses from there in either direction on the genome. Eventually, the genome is strongly methylated, except for the terminal 2 to 5% on either end, which remains hypomethylated. Similar observations have been made with tumor cell lines with different sites of Ad12 DNA integration. In contrast, the levels of DNA methylation do not seem to change after tumor cell explanation in several segments of hamster cell DNA of the unique or repetitive type. Restriction (HpaII) and Southern blot experiments were performed with selected cloned hamster cellular DNA probes. The data suggest that in the integrated foreign DNA, there exist nucleotide sequences or structures or chromatin arrangements that can be preferentially recognized by the system responsible for de novo DNA methylation in mammalian cells.
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