ArticleJournal of immunological methods2007
A fast and efficient procedure to produce scFvs specific for large macromolecular complexes.
Article in Journal of immunological methods, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Review
- Parallel evaluation of cell‑based phage display panning strategies: Optimized selection and depletion steps result in AML blast‑binding consensus antibodies.Molecular medicine reports · 2021Article
- Phage antibody library screening for the selection of novel high-affinity human single-chain variable fragment against gastrin receptor: an in silico and in vitro study.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2019Article
- A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick.RSC advances · 2018Article
- Improvement of the crystallizability and expression of an RNA crystallization chaperone.Journal of biochemistry · 2011Article
- Isolation of scFv fragments specific for monokine induced by interferon-gamma (MIG) using phage display.Journal of immunological methods · 2010Article
- The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae.Folia microbiologica · 2008Article
- SRP RNA provides the physiologically essential GTPase activation function in cotranslational protein targeting.RNA (New York, N.Y.) · 2007Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We have expanded the application of antibody phage display to a new type of antigen: ribonucleoprotein (RNP) complexes. We describe a simple and efficient method for screening antibodies specific for large intact RNPs and individual components. We also describe a fast and easy method to overcome the abundance of amber stop codons in the positive phage clones. The resulting antibodies have been used in ELISA and Western blot analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.