ArticleNeurourology and urodynamics2007
M2 mediated contractions of human bladder from organ donors is associated with an increase in urothelial muscarinic receptors.
Article in Neurourology and urodynamics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Dog and human bladders have different neurogenic and nicotinic responses in inner versus outer detrusor muscle layers.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Modulation of lower urinary tract smooth muscle contraction and relaxation by the urothelium.Naunyn-Schmiedeberg's archives of pharmacology · 2018Review
- Cascade bioassay evidence for the existence of urothelium-derived inhibitory factor in Guinea pig urinary bladder.PloS one · 2014Article
- M2muscarinic receptors inhibit cell proliferation and migration in urothelial bladder cancer cells.Cancer biology & therapy · 2014Article
- Alterations in nerve-evoked bladder contractions in a coronavirus-induced mouse model of multiple sclerosis.PloS one · 2014Article
- Elevated polyamines in urothelial cells from OAB subjects mediate oxotremorine-evoked rapid intracellular calcium rise and delayed acetylcholine release.American journal of physiology. Renal physiology · 2013Article
- Gastric body cholinergic contractile signal transduction in M2 and M3 receptor knockout mice.Journal of receptor and signal transduction research · 2013Article
- How does the urothelium affect bladder function in health and disease? ICI-RS 2011.Neurourology and urodynamics · 2012Review
- Comparison of human and porcine gastric clasp and sling fiber contraction by M2 and M3 muscarinic receptors.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Quantitation of the contractile response mediated by two receptors: M2 and M3 muscarinic receptor-mediated contractions of human gastroesophageal smooth muscle.The Journal of pharmacology and experimental therapeutics · 2009Article
- Urotheliogenic modulation of intrinsic activity in spinal cord-transected rat bladders: role of mucosal muscarinic receptors.American journal of physiology. Renal physiology · 2008Article
- The neural control of micturition.Nature reviews. Neuroscience · 2008Review
- The use of occupation isoboles for analysis of a response mediated by two receptors: M2 and M3 muscarinic receptor subtype-induced mouse stomach contractions.The Journal of pharmacology and experimental therapeutics · 2008Article
- Refinement in the management of the denervated canine urinary bladder using an abdominal vesicostomy.ILAR journal · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
aimsPrevious studies have shown increased density of M2 receptors in hypertrophied rat bladders that possess an M2 contractile phenotype. The aim of the current study is to determine whether human bladders with an M2 contractile phenotype also have a greater density of bladder M2 receptors. MATERIALS AND
methodsHuman bladders were obtained from 24 different organ transplant donors. Darifenacin and methoctramine affinity was determined by the rightward shift of cumulative carbachol concentration contractile response curves for each bladder. Radioligand binding and immunoprecipitation was used to quantify M2 and M3 subtypes in isolated detrusor muscle and urothelium. In addition, pig bladder muscle and urothelial receptors were quantified for comparison.
resultsIn the human urothelium total, M2 and M3 muscarinic receptor density is significantly negatively correlated with the affinity of darifenacin for inhibition of contraction of the detrusor muscle. In the detrusor muscle there is no correlation between receptor density and darifenacin affinity for inhibition of contraction. Muscarinic receptor density is greater in the muscle than in the urothelium in human bladders whereas in the pig bladder the density is greater in the urothelium than in the muscle.
conclusionsThe greater density of urothelial muscarinic receptors in human bladders with lower darifenacin affinity, indicative of a greater contribution of M2 receptors to the contractile response, points towards a possible role of the urothelium in controlling M2 mediated contractile phenotype. In comparison between human and pig bladders, the distribution of muscarinic receptor subtypes in the muscle and urothelium are quite different.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.