ArticleThe EMBO journal1991
Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 180 citations in OpenAlex.
- Human Colonoid-Myofibroblast Coculture for Study of Apical NaInternational journal of molecular sciences · 2023Article
- Intestinal brush-border Na+/H+ exchanger-3 drives H+-coupled iron absorption in the mouse.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Proximal nephron.Comprehensive Physiology · 2013Review
- Loss of PDZ-adaptor protein NHERF2 affects membrane localization and cGMP- and [Ca2+]- but not cAMP-dependent regulation of Na+/H+ exchanger 3 in murine intestine.The Journal of physiology · 2010Article
- Ischemic post-conditioning to counteract intestinal ischemia/reperfusion injury.World journal of gastrointestinal pathophysiology · 2010Article
- Review
- Epidermal growth factor inhibits intestinal NHE8 expression via reducing its basal transcription.American journal of physiology. Cell physiology · 2010Article
- Mechanisms of the regulation of the intestinal Na+/H+ exchanger NHE3.Journal of biomedicine & biotechnology · 2010Review
- Tumor necrosis factor-{alpha} downregulates intestinal NHE8 expression by reducing basal promoter activity.American journal of physiology. Cell physiology · 2009Article
- Proline residues in transmembrane segment IV are critical for activity, expression and targeting of the Na+/H+ exchanger isoform 1.The Biochemical journal · 2004Article
- Diversity of the mammalian sodium/proton exchanger SLC9 gene family.Pflugers Archiv : European journal of physiology · 2004Review
- Role of glucocorticoids in the maturation of the rat renal Na+/H+ antiporter (NHE3).Kidney international · 2001Article
- Novel amiloride-sensitive sodium-dependent proton secretion in the mouse proximal convoluted tubule.The Journal of clinical investigation · 2000Article
- Regulation of intracellular pH during H+-coupled oligopeptide absorption in enterocytes from guinea-pig ileum.The Journal of physiology · 1998Article
- Glucose metabolism, H+ production and Na+/H+-exchanger mRNA levels in ischemic hearts from diabetic rats.Molecular and cellular biochemistry · 1998Article
- Role of brush border Na+/H+ exchange in canine ileal absorption.Digestive diseases and sciences · 1996Article
- Na+/H+ exchange activity during phagocytosis in human neutrophils: role of Fcgamma receptors and tyrosine kinases.The Journal of cell biology · 1996Article
- Biology of membrane transport proteins.Pharmaceutical research · 1995Review
- Chimeric Na+/H+ exchangers: an epithelial membrane-bound N-terminal domain requires an epithelial cytoplasmic C-terminal domain for regulation by protein kinases.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Expression of a novel sodium-hydrogen exchanger in the gastrointestinal tract and kidney.The Journal of membrane biology · 1995Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
A cDNA clone encoding a rabbit ileal villus cell Na+/H+ exchanger was isolated and its complete nucleotide sequence was determined. The cDNA is 4 kb long and contains 322 bp of 5'-untranslated region, 2451 bp of open reading frame and 1163 bp of 3'-untranslated area, with 70%, 91% and 40% identity to the human sequence, respectively. Amino acid sequence deduced from the longest open reading frame indicated a protein of 816 residues (predicted Mr 90,716) which exhibits 95% amino acid identity to the human Na+/H+ exchanger. The two putative glycosylation sites in the human Na+/H+ exchanger are conserved in this protein, suggesting that it is a glycoprotein. Stable transfection of the cDNA into an Na+/H+ exchanger deficient fibroblast cell line, established Na+/H+ exchange. The Na+/H+ exchanger was stimulated by serum and a phorbol ester but not by 8-Br-cAMP. In Northern blot analysis, the cDNA hybridized to a 4.8 kb message in rabbit ileal villus cells, kidney cortex, kidney medulla, adrenal gland, brain and descending colon and to a 5.2 kb message in cultured human colonic cancer cell lines, HT29-18 and Caco-2. In immunoblotting, a polyclonal antibody raised against a fusion protein of beta-galactosidase and the C-terminal 158 amino acids of the human Na+/H+ exchanger identified a rabbit ileal basolateral membrane protein of 94 kd and only weakly interacted with the ileal brush border membrane. In immunocytochemical studies using ileal villus and crypt epithelial cells, the same antibody identified basolateral and not brush border epitopes. Restriction analysis of genomic DNA with a 462 bp PstI-AccI fragment of the rabbit Na+/H+ exchanger strongly suggests the existence of closely related Na+/H+ exchanger genes. The near identity of the basolateral Na+/H+ exchanger and the human Na+/H+ exchanger plus the ubiquitous expression of this message suggests that the ileal basolateral Na+/H+ exchanger is the 'housekeeping' Na+/H+ exchanger.
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