ArticleAnnals of surgery2006
Precultivation of engineered human nasal cartilage enhances the mechanical properties relevant for use in facial reconstructive surgery.
Article in Annals of surgery, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 56 citations in OpenAlex.
- 3D Bioprinting of Hyaline Cartilage Using Nasal Chondrocytes.Annals of biomedical engineering · 2024Review
- A biomimetic multilayered polymeric material designed for heart valve repair and replacement.Biomaterials · 2022Article
- Facial Cartilaginous Reconstruction-A Historical Perspective, State-of-the-Art, and Future Directions.Frontiers in surgery · 2021Review
- Evaluation of human nasal cartilage nonlinear and rate dependent mechanical properties.Journal of biomechanics · 2020Article
- Head to Knee: Cranial Neural Crest-Derived Cells as Promising Candidates for Human Cartilage Repair.Stem cells international · 2019Review
- Biomechanical evaluation of suture-holding properties of native and tissue-engineered articular cartilage.Biomechanics and modeling in mechanobiology · 2015Article
- Engineered nasal cartilage by cell homing: a model for augmentative and reconstructive rhinoplasty.Plastic and reconstructive surgery · 2014Article
- Cell therapies and regenerative medicine - the dawn of a new age or more hype than hope?Clinical and translational medicine · 2012Article
- Response of human engineered cartilage based on articular or nasal chondrocytes to interleukin-1β and low oxygen.Tissue engineering. Part A · 2012Article
- In vivo implantation of tissue-engineered human nasal septal neocartilage constructs: a pilot study.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2012Article
- Article
- Model for estimating the threshold mechanical stability of structural cartilage grafts used in rhinoplasty.The Laryngoscope · 2010Article
- Injectable biomaterials for regenerating complex craniofacial tissues.Advanced materials (Deerfield Beach, Fla.) · 2009Article
- Asymmetrical strain distributions and neutral axis location of cartilage in flexure.Journal of biomechanics · 2009Article
- In vitro maturation and in vivo stability of bioprinted human nasal cartilage.Journal of tissue engineeringArticle
- Model to Estimate Threshold Mechanical Stability of Lower Lateral Cartilage.JAMA facial plastic surgeryArticle
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate if precultivation of human engineered nasal cartilage grafts of clinically relevant size would increase the suture retention strength at implantation and the tensile and bending stiffness 2 weeks after implantation. SUMMARY BACKGROUND INFORMATION: To be used for reconstruction of nasal cartilage defects, engineered grafts need to be reliably sutured at implantation and resist to bending/tension forces about 2 weeks after surgery, when fixation is typically removed.
methodsNasal septum chondrocytes from 4 donors were expanded for 2 passages and statically loaded on 15 x 5 x 2-mm size nonwoven meshes of esterified hyaluronan (Hyaff-11). Constructs were implanted for 2 weeks in nude mice between muscle fascia and subcutaneous tissue either directly after cell seeding or after 2 or 4 weeks of preculture in chondrogenic medium. Engineered tissues and native nasal cartilage were assessed histologically, biochemically, and biomechanically.
resultsEngineered constructs reproducibly developed with culture time into cartilaginous tissues with increasing content of glycosaminoglycans and collagen type II. Suture retention strength was significantly higher (3.6 +/- 2.2-fold) in 2-week precultured constructs than in freshly seeded meshes. Following in vivo implantation, tissues further developed and maintained the original scaffold size and shape. The bending stiffness was significantly higher (1.8 +/- 0.8-fold) if constructs were precultured for 2 weeks than if they were directly implanted, whereas tensile stiffness was close to native cartilage in all groups.
conclusionIn our experimental setup, preculture for 2 weeks was necessary to engineer nasal cartilage grafts with enhanced mechanical properties relevant for clinical use in facial reconstructive surgery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.