ArticleJournal of bacteriology2007
Identification of genes regulated by the cepIR quorum-sensing system in Burkholderia cenocepacia by high-throughput screening of a random promoter library.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Harnessing hypoxia: bacterial adaptation and chronic infection in cystic fibrosis.FEMS microbiology reviews · 2025Review
- Presence of the Hmq System and Production of 4-Hydroxy-3-Methyl-2-Alkylquinolines Are Heterogeneously Distributed between Burkholderia cepacia Complex Species and More Prevalent among Environmental than Clinical Isolates.Microbiology spectrum · 2021Article
- ScmR, a Global Regulator of Gene Expression, Quorum Sensing, pH Homeostasis, and Virulence inJournal of bacteriology · 2020Article
- Quorum Sensing Controls Both Rhamnolipid and Polyhydroxyalkanoate Production inFrontiers in bioengineering and biotechnology · 2020Article
- Loss ofmSphere · 2019Article
- Quorum Sensing as Antivirulence Target in Cystic Fibrosis Pathogens.International journal of molecular sciences · 2019Review
- Various Evolutionary Trajectories Lead to Loss of the Tobramycin-Potentiating Activity of the Quorum-Sensing Inhibitor Baicalin Hydrate inAntimicrobial agents and chemotherapy · 2019Article
- TwoJournal of bacteriology · 2018Article
- The Complex Quorum Sensing Circuitry ofmBio · 2017Article
- Interplay between 4-Hydroxy-3-Methyl-2-Alkylquinoline andFrontiers in microbiology · 2017Article
- Potential of metabolomics to reveal Burkholderia cepacia complex pathogenesis and antibiotic resistance.Frontiers in microbiology · 2015Review
- Identification of quorum sensing-controlled genes in Burkholderia ambifaria.MicrobiologyOpen · 2013Article
- A unique regulator contributes to quorum sensing and virulence in Burkholderia cenocepacia.PloS one · 2012Article
- Burkholderia cenocepacia ShvR-regulated genes that influence colony morphology, biofilm formation, and virulence.Infection and immunity · 2011Article
- Saturation mutagenesis of a CepR binding site as a means to identify new quorum-regulated promoters in Burkholderia cenocepacia.Molecular microbiology · 2011Article
- Functional quorum sensing systems are maintained during chronic Burkholderia cepacia complex infections in patients with cystic fibrosis.The Journal of infectious diseases · 2011Article
- Long-term colonization of the cystic fibrosis lung by Burkholderia cepacia complex bacteria: epidemiology, clonal variation, and genome-wide expression alterations.Frontiers in cellular and infection microbiology · 2011Article
- The Burkholderia cenocepacia LysR-type transcriptional regulator ShvR influences expression of quorum-sensing, protease, type II secretion, and afc genes.Journal of bacteriology · 2011Article
- A decade of Burkholderia cenocepacia virulence determinant research.Infection and immunity · 2010Review
- Differential modulation of Burkholderia cenocepacia virulence and energy metabolism by the quorum-sensing signal BDSF and its synthase.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Burkholderia cenocepacia cepIR quorum-sensing system regulates expression of extracellular proteases, chitinase, and genes involved in ornibactin biosynthesis, biofilm formation, and motility. In a genome-wide screen we identified cepIR-regulated genes by screening a random promoter library of B. cenocepacia K56-2 constructed in a luminescence reporter detection plasmid for differential expression in response to N-octanoyl-l-homoserine lactone (OHL). Eighty-nine clones were identified; in 58 of these clones expression was positively regulated by cepIR, and in 31 expression was negatively regulated by cepIR. The expression profiles of the 89 promoter clones were compared in the cepI mutant K56-dI2 in medium supplemented with 30 pM OHL and K56-2 to confirm that the presence of OHL restored expression to wild-type levels. To validate the promoter library observations and to determine the effect of a cepR mutation on expression of selected genes, the mRNA levels of nine genes whose promoters were predicted to be regulated by cepR were quantitated by quantitative reverse transcription-PCR in the wild type and cepI and cepR mutants. The expression levels of all nine genes were similar in the cepI and cepR mutants and consistent with the promoter-lux reporter activity. The expression of four selected cepIR-regulated gene promoters was examined in a cciIR mutant, and two of these promoters were also regulated by cciIR. This study extends our understanding of genes whose expression is influenced by cepIR and indicates the global regulatory effect of the cepIR system in B. cenocepacia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.