ArticleThe Journal of biological chemistry2007
Impact of membrane fusion and proteolysis on SpoIIQ dynamics and interaction with SpoIIIAH.
Article in The Journal of biological chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Deciphering metabolic differentiation during Bacillus subtilis sporulation.Nature communications · 2025Article
- The small acid-soluble proteins of Clostridioides difficile regulate sporulation in a SpoIVB2-dependent manner.PLoS pathogens · 2024Article
- Cleavage of an engulfment peptidoglycan hydrolase by a sporulation signature protease in Clostridioides difficile.Molecular microbiology · 2024Article
- The small acid-soluble proteins ofbioRxiv : the preprint server for biology · 2024Article
- Structural, Metabolic and Evolutionary Comparison of Bacterial Endospore and Exospore Formation.Frontiers in microbiology · 2021Review
- Channels modestly impact compartment-specific ATP levels during Bacillus subtilis sporulation and a rise in the mother cell ATP level is not necessary for Pro-σMolecular microbiology · 2020Article
- A Hybrid Secretion System Facilitates Bacterial Sporulation: A Structural Perspective.Microbiology spectrum · 2019Review
- Evidence that regulation of intramembrane proteolysis is mediated by substrate gating during sporulation in Bacillus subtilis.PLoS genetics · 2018Article
- GerM is required to assemble the basal platform of the SpoIIIA-SpoIIQ transenvelope complex during sporulation in Bacillus subtilis.Molecular microbiology · 2016Article
- Complex Formed between Intramembrane Metalloprotease SpoIVFB and Its Substrate, Pro-σK.The Journal of biological chemistry · 2016Article
- A Membrane-Embedded Amino Acid Couples the SpoIIQ Channel Protein to Anti-Sigma Factor Transcriptional Repression during Bacillus subtilis Sporulation.Journal of bacteriology · 2016Article
- The SpoIIQ-SpoIIIAH complex of Clostridium difficile controls forespore engulfment and late stages of gene expression and spore morphogenesis.Molecular microbiology · 2016Article
- Regulated proteolysis in bacterial development.FEMS microbiology reviews · 2014Review
- Biochemical and structural insights into intramembrane metalloprotease mechanisms.Biochimica et biophysica acta · 2013Review
- The SpoIIQ landmark protein has different requirements for septal localization and immobilization.Molecular microbiology · 2013Article
- Genomic determinants of sporulation in Bacilli and Clostridia: towards the minimal set of sporulation-specific genes.Environmental microbiology · 2012Article
- A LytM domain dictates the localization of proteins to the mother cell-forespore interface during bacterial endospore formation.Journal of bacteriology · 2011Article
- Holin triggering in real time.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- SpoIID-mediated peptidoglycan degradation is required throughout engulfment during Bacillus subtilis sporulation.Journal of bacteriology · 2010Article
- A channel connecting the mother cell and forespore during bacterial endospore formation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The onset of engulfment-dependent gene expression during Bacillus subtilis sporulation requires the forespore membrane protein SpoIIQ, which recruits mother cell proteins involved in late gene expression to the outer forespore membrane. Engulfment activates the late forespore transcription factor sigmaG, which produces high levels of the secreted SpoIVB protease that is required for activation of the late mother cell transcription factor sigmaK. Engulfment also triggers the proteolytic cleavage of SpoIIQ, an event that depends on the SpoIVB protease but not on sigmaG activity. To determine if SpoIVB directly cleaves SpoIIQ and to determine if this event participates in the onset of late gene expression, we purified SpoIVB, SpoIIQ, and SpoIVFA (another SpoIVB substrate). SpoIVB directly cleaved SpoIIQ at the same site in vitro and in vivo and cleaved SpoIVFA in at least three different locations. SpoIIQ cleavage depends on membrane fusion, but not on sigmaG activity, suggesting that the ability of SpoIVB to cleave substrates is regulated by membrane fusion. We isolated SpoIVB-resistant SpoIIQ proteins by random mutagenesis of codons at the cleavage site and demonstrated that SpoIIQ processing is dispensable for spore formation and for activation of late forespore and mother cell gene expression. Fluorescence recovery after photobleaching analysis demonstrated that membrane fusion releases SpoIIQ from an immobile complex, an event that could allow SpoIVB to cleave SpoIIQ. We propose that this membrane fusion-dependent reorganization in the complex, rather than SpoIIQ proteolysis itself, is necessary for the onset of late transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.