ArticleAnnals of clinical microbiology and antimicrobials2006
Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species.
Article in Annals of clinical microbiology and antimicrobials, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers.
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145 citing papers in PubMed.
- A systematic review ofOne health (Amsterdam, Netherlands) · 2026Article
- Molecular detection and genetic characterisation of a large flood-borne outbreak of human leptospirosis in Jakarta, Indonesia: A retrospective analysis of surveillance data.PLoS neglected tropical diseases · 2026Article
- Epidemiological study of leptospiral interaction in bovine farms in rural areas of Colombia: A One Health approach.PLoS neglected tropical diseases · 2026Article
- Wild Boar (Animals : an open access journal from MDPI · 2026Article
- First report of pathogenic Leptospira in Sri Lankan bats: A potential reservoir risk?PLoS neglected tropical diseases · 2026Article
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- Molecular Diagnosis and Quantification of Bovine Abortion Caused byVeterinary medicine international · 2026Article
- Integrative assessment of species-level genetic markers for the diagnosis of pathogenicFrontiers in public health · 2026Article
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- High Resolution Genotyping of Leptospira Direct from Clinical Samples Using Multiplexed Amplicon Sequencing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Host-Driven Genetic Diversity ofTransboundary and emerging diseases · 2026Article
- Shaping the future ofJournal of medical microbiology · 2025Review
- Canine Leptospirosis in Flood-Affected Areas of Southern Brazil: Molecular Assessment and Public Health Implications.Infectious disease reports · 2025Article
- Identification of virulence genes and clade-specific markers through pan-genomic analysis of Leptospira.BMC microbiology · 2025Article
- Host population dynamics influence Leptospira spp. transmission patterns among Rattus norvegicus in Boston, Massachusetts, US.PLoS neglected tropical diseases · 2025Article
- Leptospirosis in Unconventional Mammal Pets.Veterinary sciences · 2025Review
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- Bacteriological and Serological Investigation of Leptospirosis in Dogs and Pigs in Palau.The American journal of tropical medicine and hygiene · 2024Article
- Molecular Characterization ofTropical medicine and infectious disease · 2024Article
- Detection of Leptospira kirschneri in a short-beaked common dolphin (Delphinus delphis delphis) stranded off the coast of southern California, USA.BMC veterinary research · 2024Article
85 more citing papers are in PubMed but not listed here.
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7 authors.
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Abstract
backgroundLeptospira are the parasitic bacterial organisms associated with a broad range of mammalian hosts and are responsible for severe cases of human Leptospirosis. The epidemiology of leptospirosis is complex and dynamic. Multiple serovars have been identified, each adapted to one or more animal hosts. Adaptation is a dynamic process that changes the spatial and temporal distribution of serovars and clinical manifestations in different hosts. Serotyping based on repertoire of surface antigens is an ambiguous and artificial system of classification of leptospiral agents. Molecular typing methods for the identification of pathogenic leptospires up to individual genome species level have been highly sought after since the decipherment of whole genome sequences. Only a few resources exist for microbial genotypic data based on individual techniques such as Multiple Locus Sequence Typing (MLST), but unfortunately no such databases are existent for leptospires.
resultsWe for the first time report development of a robust MLST method for genotyping of Leptospira. Genotyping based on DNA sequence identity of 4 housekeeping genes and 2 candidate genes was analyzed in a set of 120 strains including 41 reference strains representing different geographical areas and from different sources. Of the six selected genes, adk, icdA and secY were significantly more variable whereas the LipL32 and LipL41 coding genes and the rrs2 gene were moderately variable. The phylogenetic tree clustered the isolates according to the genome-based species.
conclusionThe main advantages of MLST over other typing methods for leptospires include reproducibility, robustness, consistency and portability. The genetic relatedness of the leptospires can be better studied by the MLST approach and can be used for molecular epidemiological and evolutionary studies and population genetics.
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