ArticlePlant molecular biology2007
Nine 3-ketoacyl-CoA thiolases (KATs) and acetoacetyl-CoA thiolases (ACATs) encoded by five genes in Arabidopsis thaliana are targeted either to peroxisomes or cytosol but not to mitochondria.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed, 110 citations in OpenAlex.
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- The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense againstInternational journal of molecular sciences · 2023Article
- Overexpression of Bacterial Beta-Ketothiolase Improves Flax (Metabolites · 2023Article
- Peroxisomal KAT2 (3-ketoacyl-CoA thiolase 2) gene has a key role in gingerol biosynthesis in ginger (Journal of plant biochemistry and biotechnology · 2023Article
- Identification and Characterization of Transcription Factors Involved in Geraniol Biosynthesis inInternational journal of molecular sciences · 2022Article
- Metabolic alterations in algaEnvironmental science. Nano · 2022Article
- Long-Chain Acyl-CoA Synthetases Promote Poplar Resistance to Abiotic Stress by Regulating Long-Chain Fatty Acid Biosynthesis.International journal of molecular sciences · 2022Article
- Molecular Cloning and Analysis of an Acetyl-CoA C-acetyltransferase Gene (Plants (Basel, Switzerland) · 2022Article
- Multilevel interactions between native and ectopic isoprenoid pathways affect global metabolism in rice.Transgenic research · 2022Article
- The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis.The Plant journal : for cell and molecular biology · 2022Article
- Identification of Six Thiolases and Their Effects on Fatty Acid and Ergosterol Biosynthesis inApplied and environmental microbiology · 2022Article
- Inferred Subcellular Localization of Peroxisomal Matrix Proteins ofFrontiers in plant science · 2022Article
- Presequence translocase-associated motor subunits of the mitochondrial protein import apparatus are dual-targeted to mitochondria and plastids.Frontiers in plant science · 2022Article
- Iron insufficiency in floral buds impairs pollen development by disrupting tapetum function.The Plant journal : for cell and molecular biology · 2021Article
- Dynamic transcriptome analysis identifies genes related to fatty acid biosynthesis in the seeds of Prunus pedunculata Pall.BMC plant biology · 2021Article
- Cloning, characterization, and functional analysis of acetyl-CoA C-acetyltransferase and 3-hydroxy-3-methylglutaryl-CoA synthase genes in Santalum album.Scientific reports · 2021Article
- Article
- The Acetate Pathway Supports Flavonoid and Lipid Biosynthesis in Arabidopsis.Plant physiology · 2020Article
- De novo transcriptome assembly for the five major organs of Zanthoxylum armatum and the identification of genes involved in terpenoid compound and fatty acid metabolism.BMC genomics · 2020Article
- Transcriptional characterization and response to defense elicitors of mevalonate pathway genes in cotton (PeerJ · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sub-cellular location of enzymes of fatty acid beta-oxidation in plants is controversial. In the current debate the role and location of particular thiolases in fatty acid degradation, fatty acid synthesis and isoleucine degradation are important. The aim of this research was to determine the sub-cellular location and hence provide information about possible functions of all the putative 3-ketoacyl-CoA thiolases (KAT) and acetoacetyl-CoA thiolases (ACAT) in Arabidopsis. Arabidopsis has three genes predicted to encode KATs, one of which encodes two polypeptides that differ at the N-terminal end. Expression in Arabidopsis cells of cDNAs encoding each of these KATs fused to green fluorescent protein (GFP) at their C-termini showed that three are targeted to peroxisomes while the fourth is apparently cytosolic. The four KATs are also predicted to have mitochondrial targeting sequences, but purified mitochondria were unable to import any of the proteins in vitro. Arabidopsis also has two genes encoding a total of five different putative ACATs. One isoform is targeted to peroxisomes as a fusion with GFP, while the others display no targeting in vivo as GFP fusions, or import into isolated mitochondria. Analysis of gene co-expression clusters in Arabidopsis suggests a role for peroxisomal KAT2 in beta-oxidation, while KAT5 co-expresses with genes of the flavonoid biosynthesis pathway and cytosolic ACAT2 clearly co-expresses with genes of the cytosolic mevalonate biosynthesis pathway. We conclude that KATs and ACATs are present in the cytosol and peroxisome, but are not found in mitochondria. The implications for fatty acid beta-oxidation and for isoleucine degradation in mitochondria are discussed.
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