ArticleJournal of the American Chemical Society2006
X-ray crystal structures of manganese(II)-reconstituted and native toluene/o-xylene monooxygenase hydroxylase reveal rotamer shifts in conserved residues and an enhanced view of the protein interior.
Article in Journal of the American Chemical Society, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Catalysis and Electron Transfer inChemical reviews · 2022Review
- Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.Chemical reviews · 2018Review
- Structure/function correlations over binuclear non-heme iron active sites.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2016Review
- Article
- Structural basis for biomolecular recognition in overlapping binding sites in a diiron enzyme system.Nature communications · 2014Article
- A flexible glutamine regulates the catalytic activity of toluene o-xylene monooxygenase.Biochemistry · 2014Article
- Gates of enzymes.Chemical reviews · 2013Review
- Conservation of the three-dimensional structure in non-homologous or unrelated proteins.Human genomics · 2012Review
- Article
- Tracking a defined route for O₂ migration in a dioxygen-activating diiron enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Insights into the different dioxygen activation pathways of methane and toluene monooxygenase hydroxylases.Journal of the American Chemical Society · 2011Article
- Determining macromolecular assembly structures by molecular docking and fitting into an electron density map.Proteins · 2010Article
- Crystallization and preliminary X-ray analysis of PaaAC, the main component of the hydroxylase of the Escherichia coli phenylacetyl-coenzyme A oxygenase complex.Acta crystallographica. Section F, Structural biology and crystallization communications · 2010Article
- Synthesis, Characterization, and Oxygenation Studies of Carboxylate-Bridged Diiron(II) Complexes with Aromatic Substrates Tethered to Pyridine Ligands and the Formation of a Unique Trinuclear Complex.European journal of inorganic chemistry · 2009Article
- Structural consequences of effector protein complex formation in a diiron hydroxylase.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Protein engineering of toluene monooxygenases for synthesis of chiral sulfoxides.Applied and environmental microbiology · 2008Article
- Dioxygen activation at non-heme diiron centers: oxidation of a proximal residue in the I100W variant of toluene/o-xylene monooxygenase hydroxylase.Biochemistry · 2007Article
- Characterization of the arene-oxidizing intermediate in ToMOH as a diiron(III) species.Journal of the American Chemical Society · 2007Article
- X-ray structure of a hydroxylase-regulatory protein complex from a hydrocarbon-oxidizing multicomponent monooxygenase, Pseudomonas sp. OX1 phenol hydroxylase.Biochemistry · 2006Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We report the X-ray crystal structures of native and manganese(II)-reconstituted toluene/o-xylene monooxygenase hydroxylase (ToMOH) from Pseudomonas stutzeri OX1 to 1.85 and 2.20 A resolution, respectively. The structures reveal that reduction of the dimetallic active site is accompanied by a carboxylate shift and alteration of the coordination environment for dioxygen binding and activation. A rotamer shift in a strategically placed asparagine 202 accompanies dimetallic center reduction and is proposed to influence protein component interactions. This rotamer shift is conserved between ToMOH and the corresponding residue in methane monooxygenase hydroxylase (MMOH). Previously unidentified hydrophobic pockets similar to those present in MMOH are assigned.
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