ArticleMolecular and cellular biology2007
A two-step, PU.1-dependent mechanism for developmentally regulated chromatin remodeling and transcription of the c-fms gene.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 100 citations in OpenAlex.
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- Synovial macrophage diversity and activation of M-CSF signaling in post-traumatic osteoarthritis.eLife · 2025Article
- Understanding blood development and leukemia using sequencing-based technologies and human cell systems.Frontiers in molecular biosciences · 2023Review
- Chromatin Remodeling of Colorectal Cancer Liver Metastasis is Mediated by an HGF-PU.1-DPP4 Axis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Beyond Immunity: Underappreciated Functions of Intestinal Macrophages.Frontiers in immunology · 2021Review
- Transcriptomic profiling of feline teeth highlights the role of matrix metalloproteinase 9 (MMP9) in tooth resorption.Scientific reports · 2020Article
- The Transcriptional Network That Controls Growth Arrest and Macrophage Differentiation in the Human Myeloid Leukemia Cell Line THP-1.Frontiers in cell and developmental biology · 2020Article
- Mechanisms of Action of Hematopoietic Transcription Factor PU.1 in Initiation of T-Cell Development.Frontiers in immunology · 2019Review
- Single-Cell Multi-omics: An Engine for New Quantitative Models of Gene Regulation.Trends in genetics : TIG · 2018Review
- Targeted Enhancer Activation by a Subunit of the Integrator Complex.Molecular cell · 2018Article
- Transformation of Accessible Chromatin and 3D Nucleome Underlies Lineage Commitment of Early T Cells.Immunity · 2018Article
- Mathematical Analysis of Cytokine-Induced Differentiation of Granulocyte-Monocyte Progenitor Cells.Frontiers in immunology · 2018Article
- PU.1 acts as tumor suppressor for myeloma cells through direct transcriptional repression of IRF4.Oncogene · 2017Article
- Logical modeling of lymphoid and myeloid cell specification and transdifferentiation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Macrophage production and activation are dependent on TRIM33.Oncotarget · 2017Article
- Protein kinase A inhibition of macrophage maturation is accompanied by an increase in DNA methylation of the colony-stimulating factor 1 receptor gene.Immunology · 2016Article
- Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Amphibian macrophage development and antiviral defenses.Developmental and comparative immunology · 2016Review
- Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation.Developmental cell · 2016Article
- Origin, development, and homeostasis of tissue-resident macrophages.Immunological reviews · 2014Review
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Hematopoietic stem cells and multipotent progenitors exhibit low-level transcription and partial chromatin reorganization of myeloid cell-specific genes including the c-fms (csf1R) locus. Expression of the c-fms gene is dependent on the Ets family transcription factor PU.1 and is upregulated during myeloid differentiation, enabling committed macrophage precursors to respond to colony-stimulating factor 1. To analyze molecular mechanisms underlying the transcriptional priming and developmental upregulation of the c-fms gene, we have utilized myeloid progenitors lacking the transcription factor PU.1. PU.1 can bind to sites in both the c-fms promoter and the c-fms intronic regulatory element (FIRE enhancer). Unlike wild-type progenitors, the PU.1(-/-) cells are unable to express c-fms or initiate macrophage differentiation. When PU.1 was reexpressed in mutant progenitors, the chromatin structure of the c-fms promoter was rapidly reorganized. In contrast, assembly of transcription factors at FIRE, acquisition of active histone marks, and high levels of c-fms transcription occurred with significantly slower kinetics. We demonstrate that the reason for this differential activation was that PU.1 was required to promote induction and binding of a secondary transcription factor, Egr-2, which is important for FIRE enhancer activity. These data suggest that the c-fms promoter is maintained in a primed state by PU.1 in progenitor cells and that at FIRE PU.1 functions with another transcription factor to direct full activation of the c-fms locus in differentiated myeloid cells. The two-step mechanism of developmental gene activation that we describe here may be utilized to regulate gene activity in a variety of developmental pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.